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Synthetic peptide corresponding to a region between residues 250 and 300 of human GPCR TGR5 (NP_733800.1).
Our Abpromise guarantee covers the use of ab72608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 35 kDa).|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 23341498|
Formalin-fixed, paraffin-embedded mouse colon tissue stained for GPCR TGR5 with ab72608 at 1/200 dilution in immunohistochemical analysis. DAB staining. Counter stained with Hematoxylin.
HEK-293 (human epithelial cell line from embryonic kidney) cells stained for GPCR TGR5 (green) using ab72608 at 1/50 dilution ICC/IF.
The nuclear counter stain is DAPI (blue). Alpha- tubulin is stained with Dylight 550 (red).
IHC image of ab72608 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72608, at 1/100 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"