概述

  • 产品名称Anti-GPCR RDC1抗体
    参阅全部 GPCR RDC1 一抗
  • 描述
    兔多克隆抗体to GPCR RDC1
  • 经测试应用适用于: IHC-Fr, Flow Cyt, WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Horse, Cow, Human, Chimpanzee, Monkey
    预测可用于: Chicken, Xenopus laevis
  • 免疫原

    Synthetic peptide:

    QHNQWPMGELTC

    , corresponding to amino acids 106-117 of Human GPCR RDC1

  • 阳性对照
    • Jurkat cell lysate; human tonsil tissue.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 0.05% BSA, PBS
  • Concentration information loading...
  • 纯度Protein G purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab72100 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use a concentration of 10 µg/ml. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 4 - 6 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 42 kDa).
IHC-P Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 22745793

靶标

  • 功能Receptor for CXCL12/SDF1. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates.
  • 组织特异性Expressed in monocytes, basophils, and B-cells. Lower expression in CD4+ T-lymphocytes and natural killer cells.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • ACKR3 antibody
    • atypical chemokine receptor 3 antibody
    • C X C chemokine receptor type 7 antibody
    • C-X-C chemokine receptor type 7 antibody
    • Chemokine (C-X-C motif) receptor 7 antibody
    • Chemokine C X C motif receptor 7 antibody
    • Chemokine orphan receptor 1 antibody
    • CMKOR1 antibody
    • CXC R7 antibody
    • CXC-R7 antibody
    • CXCR 7 antibody
    • CXCR-7 antibody
    • CXCR7 antibody
    • CXCR7_HUMAN antibody
    • G protein coupled receptor 159 antibody
    • G protein coupled receptor antibody
    • G protein coupled receptor RDC1 homolog antibody
    • G-protein coupled receptor 159 antibody
    • G-protein coupled receptor RDC1 homolog antibody
    • GPR159 antibody
    • GPRN1 antibody
    • RDC 1 antibody
    • RDC-1 antibody
    • RDC1 antibody
    see all

Anti-GPCR RDC1 antibody 图像

  • Immunohistochemical analysis of GPCR RDC1 in paraffin-embedded formalin-fixed human tonsil tissue using ab72100 at 10 ug/ml
  • All lanes : Anti-GPCR RDC1 antibody (ab72100) at 5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with immunizing peptide


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
    Additional bands at : 29 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of Human bone marrow-derived Multipotent Stromal Cells staining GPCR RDC1 (green) with ab72100.

    Human MSC were treated with poly(I:C) for 6 hours before staining with primary antibody and an AlexaFluor®488-conjugated secondary antibody. Nuclei were stained with DAPI (blue).

Anti-GPCR RDC1 antibody (ab72100)参考文献

This product has been referenced in:
  • Melo Rde C  et al. CXCR7 is highly expressed in acute lymphoblastic leukemia and potentiates CXCR4 response to CXCL12. PLoS One 9:e85926 (2014). WB, Flow Cyt ; Human . Read more (PubMed: 24497931) »
  • Roy I  et al. CXCL12 chemokine expression suppresses human pancreatic cancer growth and metastasis. PLoS One 9:e90400 (2014). IHC-P ; Human . Read more (PubMed: 24594697) »

See all 12 Publications for this product

Product Wall

Thank you for your enquiry.

I have attached the requested Certificate of Compliance.

Please let me know if you need anything further.

Thanks for your email.

I can generatea Certificate of Compliance for ab72100. Is this acceptable?

Thank you for contacting us.

I am happy to let you know that we have the following antibodies available that we can guarantee to work in IHC on paraffin-embedded tissue with human samples:

CXCR4
Rabbit polyclonal ab2074:
Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain tissue)
Specification Brain tissue
Fixative Acetone/ Methanol 1:1
Permeabilization No
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Nov 18 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"