GPCR RCD1 is glycosylated so it is not unexpected that it runs higher than predicted (based on primary structure) in SDS PAGE.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR RDC1 antibody (ab38089)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
ab38089 staining pancreas (human and mouse) kidney (mouse and rat) and testis (rat and human) tissue sections by IHC-P. Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval in citrate buffer (pH 6) prior to blocking in sequential (prediluted) peroxidase and protein block for 20 minutes at 20°C. The primary antibody was diluted 1/100 and incubated with the samples for 45 minutes at 20°C. A HRP-conjugated goat anti-rabbit anitbody was used as the secondary.
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Albee LJ et al. a1-Adrenergic Receptors Function Within Hetero-Oligomeric Complexes With Atypical Chemokine Receptor 3 and Chemokine (C-X-C motif) Receptor 4 in Vascular Smooth Muscle Cells. J Am Heart Assoc6:N/A (2017).
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