概述

  • 产品名称
    Anti-GPCR GPR81抗体
    参阅全部 GPCR GPR81 一抗
  • 描述
    山羊多克隆抗体to GPCR GPR81
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Dog, Pig
  • 免疫原

    Synthetic peptide:

    C-QQLARQARMKKATR

    , corresponding to internal sequence amino acids 207-220 of Human GPCR GPR81 (NP_115943.1).

  • 阳性对照
    • Human liver lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106942 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40, 55 kDa (predicted molecular weight: 39 kDa).

靶标

  • 功能
    Acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway.
  • 组织特异性
    Expressed abundantly in brown and white fat. It also detectable at lower levels in liver, kidney, skeletal muscle, brain and pituitary. Not detected in frontal, temporal and occipital lobes of the cortex, basal forebrain, caudate nucleus, nucleus accumbens and hippocampus.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 形式
    G protein-coupled receptors (GPCRs, or GPRs), such as GPR81, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins.
  • 别名
    • FKSG80 antibody
    • FKSG80 protein antibody
    • G protein coupled receptor 104 antibody
    • G protein coupled receptor 81 antibody
    • G protein coupled receptor GPR81 antibody
    • G-protein coupled receptor 104 antibody
    • G-protein coupled receptor 81 antibody
    • GPR 104 antibody
    • GPR 81 antibody
    • GPR104 antibody
    • GPR81 antibody
    • GPR81_HUMAN antibody
    • HCA1 antibody
    • HCAR1 antibody
    • hydroxy-carboxylic acid receptor 1 antibody
    • Hydroxycarboxylic acid receptor 1 antibody
    • LACR1 antibody
    • lactate receptor 1 antibody
    • Putative chemokine receptor antibody
    • T cell activation G protein coupled receptor antibody
    • TA GPCR antibody
    see all

图片

  • Anti-GPCR GPR81 antibody (ab106942) at 0.5 µg/ml + Human liver lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 39 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 hourAn additional band of unknown identity was also consistently observed at 55 kDa. This band was successfully blocked by incubation with the immunizing peptide.

实验方案

文献

This product has been referenced in:
  • Wagner W  et al. L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation. Cell Commun Signal 13:36 (2015). WB ; Human . Read more (PubMed: 26208712) »

See 1 Publication for this product

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