Anti-GPCR GPR81抗体(ab106942)
Key features and details
- Goat polyclonal to GPCR GPR81
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GPCR GPR81抗体 -
描述
山羊多克隆抗体to GPCR GPR81 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Dog, Pig -
免疫原
Synthetic peptide:
C-QQLARQARMKKATR
, corresponding to internal sequence amino acids 207-220 of Human GPCR GPR81 (NP_115943.1). -
阳性对照
- Human liver lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab106942于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40, 55 kDa (predicted molecular weight: 39 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 40, 55 kDa (predicted molecular weight: 39 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Acts as a receptor for L-lactate and mediates its anti-lipolytic effect through a G(i)-protein-mediated pathway. -
组织特异性
Expressed abundantly in brown and white fat. It also detectable at lower levels in liver, kidney, skeletal muscle, brain and pituitary. Not detected in frontal, temporal and occipital lobes of the cortex, basal forebrain, caudate nucleus, nucleus accumbens and hippocampus. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 27198 Human
- Omim: 606923 Human
- SwissProt: Q9BXC0 Human
- Unigene: 610873 Human
- Unigene: 711001 Human
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形式
G protein-coupled receptors (GPCRs, or GPRs), such as GPR81, contain 7 transmembrane domains and transduce extracellular signals through heterotrimeric G proteins. -
别名
- FKSG80 antibody
- FKSG80 protein antibody
- G protein coupled receptor 104 antibody
see all
图片
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Anti-GPCR GPR81 antibody (ab106942) at 0.5 µg/ml + Human liver lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 hour
An additional band of unknown identity was also consistently observed at 55 kDa. This band was successfully blocked by incubation with the immunizing peptide.
数据表及文件
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Datasheet download
文献 (2)
ab106942 被引用在 2 文献中.
- Jia Q et al. Effects of GPR81 silencing combined with cisplatin stimulation on biological function in hypopharyngeal squamous cell carcinoma. Mol Med Rep 22:1727-1736 (2020). PubMed: 32582969
- Wagner W et al. L- and D-lactate enhance DNA repair and modulate the resistance of cervical carcinoma cells to anticancer drugs via histone deacetylase inhibition and hydroxycarboxylic acid receptor 1 activation. Cell Commun Signal 13:36 (2015). WB ; Human . PubMed: 26208712