兔多克隆抗体to GPCR GPR78
antigen sequence, corresponding to amino acids 261-363 of Human GPCR GPR78.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform heat mediated antigen retrieval at pH6 before commencing with IHC staining protocol.
Orphan receptor. Displays a significant level of constitutive activity. Its effect is mediated by G(s)-alpha protein that stimulate adenylate cyclase, resulting in an elevation of intracellular cAMP.
High level of expression in placenta. Expressed throughout the brain at low level. No expression detected in skeletal muscle, lung, heart, liver, pancreas, or kidney.
Belongs to the G-protein coupled receptor 1 family.
Information by UniProt
G protein coupled receptor GPR78 antibody
G-protein coupled receptor 78 antibody
Anti-GPCR GPR78 antibody 图像
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR78 antibody (ab121390)
ab121390, at 1/25 dilution, staining GPCR GPR78 in paraffin-embedded Human pancreas tissue by Immunohistochemistry, showing distinct cytoplasmic and membranous positivity in intercalated ducts.
Western blot - Anti-GPCR GPR78 antibody (ab121390)
Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
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