Anti-GPCR GPR75抗体(ab75581)

Submit an Abreview Q&A (1)

概述

  • 产品名称
    Anti-GPCR GPR75抗体
    参阅全部 GPCR GPR75 一抗
  • 描述
    兔多克隆抗体to GPCR GPR75
  • 宿主
    Rabbit
  • 特异性
    ab75581 detects endogenous levels of total GPCR GPR75 protein.
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence of human GPCR GPR75.

  • 阳性对照
    • WB: Extracts from HT-29 and K562 cells ICC/IF: LOVO cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab75581 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75581 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 59 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能
    Orphan receptor.
  • 组织特异性
    Expressed at high levels in brain and spinal cord and at detectable levels in retinal pigment epithelium. In situ hybridization of adult eye sections localized transcripts only to the perivascular cells, surrounding retinal arterioles, in the ganglion cell/nerve fiber layer.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
    • 别名
      • G protein coupled receptor 75 antibody
      • GPR chr2 antibody
      • Gpr75 antibody
      • GPR75_HUMAN antibody
      • GPRchr2 antibody
      • OTTHUMP00000159608 antibody
      • Probable G protein coupled receptor 75 antibody
      • Probable G-protein coupled receptor 75 antibody
      • WI 31133 antibody
      • WI31133 antibody
      see all

    图片

    • Western blot - GPCR GPR75 antibody (ab75581)
      Western blot - GPCR GPR75 antibody (ab75581)
      All lanes : Anti-GPCR GPR75 antibody (ab75581) at 1/500 dilution

      Lane 1 : Extracts from HT-29 cells
      Lane 2 : Extracts from K562 cells
      Lane 3 : Extracts from HT-29 cells with Immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 59 kDa
      Observed band size: 59 kDa

    • Immunocytochemistry/ Immunofluorescence - GPCR GPR75 antibody (ab75581)
      Immunocytochemistry/ Immunofluorescence - GPCR GPR75 antibody (ab75581)
      ab75581, at a 1/500 dilution, staining GPCR GPR75 in LOVO cells by Immunofluorescence, in the absence (left image) and presence (right image) of the immunising peptide.

    文献

    ab75581 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab75581？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

    提交评价 提交问题

    1 Review or Q&A

    10
    Votes: Highest First

    Customer called to ask if the region against which this product is against is intracelluar or extracelluar.

    Thank you for contacting us.

    I have been able to contact the lab regarding your question about ab75581.This antibody binds to the cytoplasmic domain of target protein.

    I hope this information is helpful to you. Please do not h...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册