概述

  • 产品名称
    Anti-GPCR GPR64抗体
    参阅全部 GPCR GPR64 一抗
  • 描述
    小鼠多克隆抗体to GPCR GPR64
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment:

    IDMPPQSETISSPMPQTHVS

    , corresponding to amino acids 313-333 of Human GPCR GPR64. This antibody was generated from mice that were immunized with mixed peptides from different regions of the antigen sequence. Serum was tested on individual peptide.

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度
    Whole antiserum
  • Primary antibody说明
    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 112 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性
    This orphan B G-protein coupled receptor could be involved in a signal transduction pathway controlling epidymal function and male fertility: it has been reported in the epididymis. ESTs have been isolated from embryo, kidney, placenta, skeletal muscle and testis libraries.
  • 细胞定位
    Cell membrane; Multi pass membrane protein.
  • 数据库链接
  • 别名
    • Epididymis specific protein 6 antibody
    • FLJ00282 antibody
    • G protein coupled receptor 64 antibody
    • G protein coupled receptor epididymis specific antibody
    • GPR 64 antibody
    • GPR64 antibody
    • HE 6 antibody
    • He 6 receptor antibody
    • HE6 antibody
    • He6 receptor antibody
    • MGC104454 antibody
    • MGC138738 antibody
    • MGC138739 antibody
    • TM7LN2 antibody
    see all

图片

  • All lanes : Anti-GPCR GPR64 antibody (ab52802) at 1/1000 dilution

    Lane 1 : Negative control lane: ~20µg of a total protein extract from E.coli with ~50ng to 100 ng of a fusion protein of an irrelevant antigen.
    Lane 2 : Test lane: ~20µg of a total protein extract from E.coli with ~50ng to 500ng of the antigen (a fusion protein).

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 112 kDa
    Observed band size : 32.2 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab52802 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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