概述

  • 产品名称Anti-GPCR GPR64抗体
    参阅全部 GPCR GPR64 一抗
  • 描述
    小鼠多克隆抗体to GPCR GPR64
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment corresponding to Human GPCR GPR64. Recombinant fragment corresponding to amino acids (100 fused) 123-142, 242-261, 275-294, 313-332, 570-589 of Human GPCR GPR64.
    Sequence:

    IMFQYDKEST VPQNQHITNG CLADHPRGPP FSSSQSIPVV SFAEPPDYSP VTHNVPSPIG IDMPPQSETI SSPMPQTHVS WDLGRNGGRG GWSDNGCSVK

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 50% Glycerol, Whole serum
  • 纯度Whole antiserum
  • Primary antibody说明This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab52801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 112 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

靶标

  • 相关性This orphan B G-protein coupled receptor could be involved in a signal transduction pathway controlling epidymal function and male fertility: it has been reported in the epididymis. ESTs have been isolated from embryo, kidney, placenta, skeletal muscle and testis libraries.
  • 细胞定位Cell membrane; Multi pass membrane protein.
  • 数据库链接
  • 别名
    • Epididymis specific protein 6 antibody
    • FLJ00282 antibody
    • G protein coupled receptor 64 antibody
    • G protein coupled receptor epididymis specific antibody
    • GPR 64 antibody
    • GPR64 antibody
    • HE 6 antibody
    • He 6 receptor antibody
    • HE6 antibody
    • He6 receptor antibody
    • MGC104454 antibody
    • MGC138738 antibody
    • MGC138739 antibody
    • TM7LN2 antibody
    see all

Anti-GPCR GPR64 antibody 图像

  • All lanes : Anti-GPCR GPR64 antibody (ab52801) at 1/1000 dilution

    Lane 1 : Negative control lane: ~20µg of a total protein extract from E.coli with ~50ng to 100 ng of a fusion protein of an irrelevant antigen.
    Lane 2 : Test lane: ~20µg of a total protein extract from E.coli with ~50ng to 500ng of the antigen (a fusion protein).

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 112 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

Anti-GPCR GPR64 antibody (ab52801)参考文献

ab52801 has not yet been referenced specifically in any publications.

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