兔多克隆抗体to GPCR GPR63
antigen sequence, corresponding to amino acids 16-81 of Human GPCR GPR63 (UniProt ID: Q9BZJ6).
WB: RT-4, U-251 MG, Human plasma, liver and tonsil
IHC-P: Human cerebellum
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Preservative: 0.02% Sodium azide Constituents: 59% PBS, 40% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use buffer at pH 6 for antigen retrieval.
1/250 - 1/500.
Orphan receptor. May play a role in brain function.
Expressed in brain; detected in the frontal cortex, with lower levels in the thalamus, caudate, hypothalamus and midbrain.
Belongs to the G-protein coupled receptor 1 family.
Information by UniProt
Brain expressed G-protein-coupled receptor PSP24 beta antibody
G protein-coupled receptor 63 antibody
G Protein-Coupled Receptor GPR63 antibody
Western blot - Anti-GPCR GPR63 antibody (ab122532)
All lanes : Anti-GPCR GPR63 antibody (ab122532) at 1/250 dilution Lane 1 : Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2 : RT-4 Lane 3 : U-251 MG Lane 4 : Human plasma Lane 5 : Human liver Lane 6 : Human tonsil Developed using the ECL technique.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR63 antibody (ab122532)
Immunohistochemical staining of GPCR GPR63 in paraffin embedded Human cerebellum tissue by Immunohistochemistry, using ab122532 at a 1/300 dilution. The image shows strong cytoplasmic positivity in Purkinje cells.
has not yet been referenced specifically in any publications.
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