概述

  • 产品名称Anti-GPCR GPR6抗体
  • 描述
    兔多克隆抗体to GPCR GPR6
  • 经测试应用适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 1-50 (NASAASLNDS QVVVVAAEGA AAAATAAGGP DTGEWGPPAA AALGAGGGAN) of Human GPCR GPR6 (NP_005275).

  • 阳性对照
    • Placenta lysate

性能

应用

Our Abpromise guarantee covers the use of ab85005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 38 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

靶标

Anti-GPCR GPR6 antibody 图像

  • Anti-GPCR GPR6 antibody (ab85005) at 1 µg/ml + 10 µg Placenta lysate

    Secondary
    HRP conjugated anti-Rabbit IgG diluted 1/50,000.

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • ICC/IF image of ab85005 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85005, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling GPCR with ab85005 at 5.0µg/ml.

Anti-GPCR GPR6 antibody (ab85005)参考文献

ab85005 has not yet been referenced specifically in any publications.

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