All lanes : Anti-GPCR GPR52 antibody (ab77513) at 1/500 dilution
Lane 1 : Extracts from HeLa cells Lane 2 : Extracts from HepG2 cells Lane 3 : Extracts from COLO cells Lane 4 : Extracts from HUVEC cells Lane 5 : Extracts from COLO cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size : 41 kDa Observed band size : 41 kDa Additional bands at : 48 kDa. We are unsure as to the identity of these extra bands.