RabMAb

Anti-GPCR GPR49抗体[EPR3065Y] (ab75850)

概述

  • 产品名称Anti-GPCR GPR49抗体[EPR3065Y]
    参阅全部 GPCR GPR49 一抗
  • 描述
    兔单克隆抗体[EPR3065Y] to GPCR GPR49
  • 经测试应用适用于: WB, IPmore details
    不适用于: Flow Cyt,ICC/IF or IHC-P
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GPCR GPR49 aa 800-900 (C terminal).

  • 阳性对照
    • Fetal skeletal muscle lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

应用

Our Abpromise guarantee covers the use of ab75850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 100 kDa.
IP 1/20.
  • 应用说明Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • 靶标

    • 功能Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
    • 组织特异性Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
    • 序列相似性Belongs to the G-protein coupled receptor 1 family.
      Contains 16 LRR (leucine-rich) repeats.
      Contains 1 LRRNT domain.
    • 细胞定位Cell membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • FEX antibody
      • G protein coupled receptor 49 antibody
      • G protein coupled receptor 67 antibody
      • g protein-coupled receptor fex antibody
      • G-protein coupled receptor 49 antibody
      • G-protein coupled receptor 67 antibody
      • G-protein coupled receptor HG38 antibody
      • GPR 49 antibody
      • GPR 67 antibody
      • GPR49 antibody
      • GPR67 antibody
      • GRP 49 antibody
      • GRP49 antibody
      • HG 38 antibody
      • HG38 antibody
      • Leucine rich repeat containing G protein coupled receptor 5 antibody
      • Leucine-rich repeat-containing G-protein coupled receptor 5 antibody
      • LGR 5 antibody
      • LGR5 antibody
      • LGR5_HUMAN antibody
      • MGC117008 antibody
      • Orphan G protein coupled receptor HG38 antibody
      see all

    Anti-GPCR GPR49 antibody [EPR3065Y] 图像

    • Anti-GPCR GPR49 antibody [EPR3065Y] (ab75850) at 1/1000 dilution + Fetal skeletal muscle lysate at 10 µg

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 100 kDa
      Observed band size : 100 kDa

    Anti-GPCR GPR49 antibody [EPR3065Y] (ab75850)参考文献

    This product has been referenced in:
    • Kantara C  et al. Methods for detecting circulating cancer stem cells (CCSCs) as a novel approach for diagnosis of colon cancer relapse/metastasis. Lab Invest 95:100-12 (2015). Read more (PubMed: 25347154) »
    • Oshima N  et al. Induction of cancer stem cell properties in colon cancer cells by defined factors. PLoS One 9:e101735 (2014). Flow Cyt ; Human . Read more (PubMed: 25006808) »

    See all 13 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Colon)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization Yes - BSA
    Specification Colon
    Blocking step Hydrogen peroxide as blocking agent for 15 minute(s) · Concentration: 3%
    Fixative Formaldehyde
    Username

    Dr. Vinicius Kannen Cardoso

    Verified customer

    提交于 Sep 09 2016

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunohistochemistry (Frozen sections)
    Sample Mouse Tissue sections (Skin)
    Permeabilization No
    Specification Skin
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative Paraformaldehyde
    Username

    Ahmar Aziz

    Verified customer

    提交于 Dec 24 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Fixation none
    Permeabilization No
    Sample Mouse Cell (colon)
    Specification colon
    Gating Strategy High FSC/SSC log scale
    Preparation Cell harvesting/tissue preparation method: isolate colon, collagenase digestion
    Sample buffer: DMEM
    Username

    Abcam user community

    Verified customer

    提交于 Aug 20 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (SW620 Colon)
    Loading amount 100 µg
    Specification SW620 Colon
    Treatment siRNA - knockdown
    Gel Running Conditions Reduced Denaturing (7)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 08 2013


    Protein G beads were indeed used with ab75850 following this protocol for IP.


    I have contacted the lab and ab75850 tested positive in IP (image attached). Rat heart is the only sample we have tested. Our recommended IP protocol is described at the following link: http://www.epitomics.com/products/support/ip, and is the same...

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    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Intestine)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM Citrate
    Permeabilization No
    Specification Intestine
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    提交于 Feb 12 2013

    Thank you for your enquiry.

    I can confirm that ab75850 Anti-GPCR GPR49 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a con...

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    Thank you for your reply.

    I have gone ahead and processed your request for a free of charge replacement antibody and you should have it by 10.30am tomorrow morning. Your new order number is 1126740.

    If there is anything else I can h...

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    Thank you for providing that information.

    Do you wish to recieve another vial of ab75850, or do wish to reciece a different antibody instead? Once I hear back from you, I will arrange the repalcement antibody to be sent as soon as possible. <...

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    1-10 of 23 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"