Anti-GPCR GPR49抗体(ab71225)

概述

  • 产品名称Anti-GPCR GPR49抗体
    参阅全部 GPCR GPR49 一抗
  • 描述
    兔多克隆抗体to GPCR GPR49
  • 经测试应用适用于: IHC-P, ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human GPCR GPR49 aa 443-473 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 8549
    (Peptide available as ab173002)

  • 阳性对照
    • K562 cell line lysates.

性能

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应用

Our Abpromise guarantee covers the use of ab71225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/50.
ICC/IF Use a concentration of 5 µg/ml.
ELISA 1/1000.
WB 1/50 - 1/100. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).

靶标

  • 功能Orphan receptor. Stem cell marker of the intestinal epithelium and the hair follicule. Target gene of Wnt signaling.
  • 组织特异性Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
    Contains 16 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FEX antibody
    • G protein coupled receptor 49 antibody
    • G protein coupled receptor 67 antibody
    • g protein-coupled receptor fex antibody
    • G-protein coupled receptor 49 antibody
    • G-protein coupled receptor 67 antibody
    • G-protein coupled receptor HG38 antibody
    • GPR 49 antibody
    • GPR 67 antibody
    • GPR49 antibody
    • GPR67 antibody
    • GRP 49 antibody
    • GRP49 antibody
    • HG 38 antibody
    • HG38 antibody
    • Leucine rich repeat containing G protein coupled receptor 5 antibody
    • Leucine-rich repeat-containing G-protein coupled receptor 5 antibody
    • LGR 5 antibody
    • LGR5 antibody
    • LGR5_HUMAN antibody
    • MGC117008 antibody
    • Orphan G protein coupled receptor HG38 antibody
    see all

Anti-GPCR GPR49 antibody 图像

  • Anti-GPCR GPR49 antibody (ab71225) at 1/1000 dilution + K562 cell line lysates at 35 µg

    Secondary
    HRP goat-anti rabbit (H+L) at 1/5000 dilution

    Predicted band size : 100 kDa
    Observed band size : 100 kDa
    Additional bands at : 55 kDa,60 kDa,72 kDa. We are unsure as to the identity of these extra bands.

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical staining of GPCR GPR49 in Human brain tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab71225 at a dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C. Antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A HRP-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • ICC/IF image of ab71225 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71225, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GPCR GPR49 antibody (ab71225)参考文献

ab71225 has not yet been referenced specifically in any publications.

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You can choose any of these, all of them should work in IHC-P/ human tissues.

We unfortunately haven't tested these antibodies in particular with intestine tissue sections however as these are tested posit...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (colon)
Specification colon
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer (Ventana CC2)
Permeabilization No
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提交于 Feb 24 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"