Anti-GPCR GPR48抗体(ab75501)

概述

  • 产品名称
    Anti-GPCR GPR48抗体
    参阅全部 GPCR GPR48 一抗
  • 描述
    兔多克隆抗体to GPCR GPR48
  • 特异性
    Detects endogenous levels of total GPCR GPR48 protein.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from an internal region of human GPCR GPR48

  • 阳性对照
    • Jurkat cell extract

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75501 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 104 kDa (predicted molecular weight: 104 kDa).
ELISA 1/5000.

靶标

  • 功能
    Orphan receptor.
  • 组织特异性
    Expressed in multiple steroidogenic tissues: placenta, ovary, testis and adrenal. Expressed also in spinal cord, thyroid, stomach, trachea, heart, pancreas, kidney, prostate and spleen.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
    Contains 15 LRR (leucine-rich) repeats.
    Contains 1 LRRNT domain.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BNMD17 antibody
    • G protein coupled receptor 48 antibody
    • G-protein coupled receptor 48 antibody
    • GPR48 antibody
    • Leucine-rich repeat-containing G protein-coupled receptor 4 antibody
    • Leucine-rich repeat-containing G-protein coupled receptor 4 antibody
    • LGR4 antibody
    • LGR4_HUMAN antibody
    see all

图片

  • All lanes : Anti-GPCR GPR48 antibody (ab75501) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : Jurkat cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 104 kDa
    Observed band size : 104 kDa

实验方案

文献

ab75501 has not yet been referenced specifically in any publications.

客户评价及客户问答

Thank you for your phone call. Please find the western blot protocol for use with ab75501 and ab75732 attached. For maximum signal, we recommend the overnight incubation at 4C with the primary antibody. Ab75501 should be used at a dilution bet...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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