概述

  • 产品名称
    Anti-GPCR GPR32抗体
    参阅全部 GPCR GPR32 一抗
  • 描述
    兔多克隆抗体to GPCR GPR32
  • 经测试应用
    适用于: Flow Cyt, WB, ICC/IF, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human GPCR GPR32 (extracellular).

  • 阳性对照
    • A549 cells

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab79516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/250. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

靶标

图片

  • All lanes : Anti-GPCR GPR32 antibody (ab79516) at 1/500 dilution

    Lane 1 : Extracts from A549 cells
    Lane 2 : Extracts from A549 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Left panel: immunofluorescence analysis of A549 cells using ab79516 at a 1/500 dilution. Right panel: same, after pre-incubation with the immunizing peptide.
  • ab79516 staining GPCR GPR32 in Human primary small airway epithelial cells by Flow Cytometry. Cells were fixed with formaldehyde and incubated with the primary antibody (1/250) for 30 minutes at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/800) was used as the secondary antibody.
    Gating Strategy: Forward and side scatter.

    See Abreview

文献

This product has been referenced in:
  • Hsiao HM  et al. Resolvin D1 attenuates polyinosinic-polycytidylic acid-induced inflammatory signaling in human airway epithelial cells via TAK1. J Immunol 193:4980-7 (2014). Read more (PubMed: 25320283) »

See 1 Publication for this product

客户评价及客户问答

Application
Flow Cytometry
Fixation
Formaldehyde
Sample
Human Cell (Human Primary Small Airway epithelial cell)
Specification
Human Primary Small Airway epithelial cell
Gating Strategy
Forward and side scatter
Permeabilization
No
Username

Dr. Jim Hsiao

Verified customer

提交于 May 15 2014

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10)
Sample
Human Cell lysate - whole cell (Human Primary Lung Fibroblasts)
Specification
Human Primary Lung Fibroblasts
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

提交于 May 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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