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Our Abpromise guarantee covers the use of ab79516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/250. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
|WB||1/500 - 1/1000. Predicted molecular weight: 40 kDa.|
|ICC/IF||1/500 - 1/1000.|
ab79516 staining GPCR GPR32 in Human primary small airway epithelial cells by Flow Cytometry. Cells were fixed with formaldehyde and incubated with the primary antibody (1/250) for 30 minutes at 4°C. An Alexa Fluor® 647-conjugated goat anti-rabbit IgG (1/800) was used as the secondary antibody.
Gating Strategy: Forward and side scatter.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"