兔多克隆抗体to GPCR GPR20
ab75559 detects endogenous levels of total GPCR GPR20 protein.
Synthesized peptide derived from an internal sequence of human GPCR GPR20
- IHC-P: human brain tissue
WB: extracts from LOVO cells
ICC/IF: A549 cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/500 - 1/1000. Predicted molecular weight: 38 kDa.
||1/50 - 1/100.
||1/500 - 1/1000.
Orphan receptor with constitutive G(i) signaling activity that activate cyclic AMP.
Ubiquitous with highest levels in intestinal tissues. In the brain detected in thalamus, putamen, and caudate, but not in frontal cortex, pons and hypothalamus.
Belongs to the G-protein coupled receptor 1 family.
Information by UniProt
- G protein coupled receptor 20 antibody
- G-protein coupled receptor 20 antibody
- Gpr20 antibody
- GPR20_HUMAN antibody
Western blot - Anti-GPCR GPR20 antibody (ab75559)
All lanes :
Anti-GPCR GPR20 antibody (ab75559) at 1/500 dilutionLane 1 :
extracts from LOVO cellsLane 2 :
extracts from LOVO cells with immunizing peptide at 5 µg
Lysates/proteins at 20 µg per lane.Predicted band size:
38 kDaObserved band size:
42 kDa (why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPCR GPR20 antibody (ab75559)
Left panel: immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.
Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR20 antibody (ab75559)
Left panel: immunofluorescence analysis of A549 cells, using 1/500 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.
has not yet been referenced specifically in any publications.
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