Anti-GPCR GPR20抗体(ab75559)
Key features and details
- Rabbit polyclonal to GPCR GPR20
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GPCR GPR20抗体 -
描述
兔多克隆抗体to GPCR GPR20 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthesized peptide derived from an internal sequence of human GPCR GPR20
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阳性对照
- IHC-P: human brain tissue WB: extracts from LOVO cells ICC/IF: A549 cells
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75559于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 38 kDa.
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IHC-P |
1/50 - 1/100.
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ICC/IF |
1/500 - 1/1000.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 38 kDa. |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Orphan receptor with constitutive G(i) signaling activity that activate cyclic AMP. -
组织特异性
Ubiquitous with highest levels in intestinal tissues. In the brain detected in thalamus, putamen, and caudate, but not in frontal cortex, pons and hypothalamus. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2843 Human
- Omim: 601908 Human
- SwissProt: Q99678 Human
- Unigene: 188859 Human
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别名
- G protein coupled receptor 20 antibody
- G-protein coupled receptor 20 antibody
- Gpr20 antibody
- GPR20_HUMAN antibody
图片
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All lanes : Anti-GPCR GPR20 antibody (ab75559) at 1/500 dilution
Lane 1 : extracts from LOVO cells
Lane 2 : extracts from LOVO cells with immunizing peptide at 5 µg
Lysates/proteins at 20 µg per lane.
Predicted band size: 38 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted? -
Left panel: immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.
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Left panel: immunofluorescence analysis of A549 cells, using 1/500 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab75559 尚未被引用在任何文献中。