概述

  • 产品名称Anti-GPCR GPR20抗体
    参阅全部 GPCR GPR20 一抗
  • 描述
    兔多克隆抗体to GPCR GPR20
  • 特异性ab75559 detects endogenous levels of total GPCR GPR20 protein.
  • 经测试应用适用于: WB, ELISA, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthesized peptide derived from an internal sequence of human GPCR GPR20

  • 阳性对照
    • IHC-P: human brain tissue WB: extracts from LOVO cells ICC/IF: A549 cells

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 38 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

靶标

  • 功能Orphan receptor with constitutive G(i) signaling activity that activate cyclic AMP.
  • 组织特异性Ubiquitous with highest levels in intestinal tissues. In the brain detected in thalamus, putamen, and caudate, but not in frontal cortex, pons and hypothalamus.
  • 序列相似性Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • G protein coupled receptor 20 antibody
    • G-protein coupled receptor 20 antibody
    • Gpr20 antibody
    • GPR20_HUMAN antibody

Anti-GPCR GPR20 antibody 图像

  • All lanes : Anti-GPCR GPR20 antibody (ab75559) at 1/500 dilution

    Lane 1 : extracts from LOVO cells
    Lane 2 : extracts from LOVO cells with immunizing peptide at 5 µg

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 38 kDa
    Observed band size : 42 kDa (why is the actual band size different from the predicted?)
  • Left panel: immunohistochemistry analysis of paraffin-embedded human brain tissue using 1/50 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.
  • Left panel: immunofluorescence analysis of A549 cells, using 1/500 ab75559; Right panel: same, after pre-incubation with the immunizing peptide.

Anti-GPCR GPR20 antibody (ab75559)参考文献

ab75559 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab75559.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"