Anti-GPCR GPR15抗体(ab16445)

概述

  • 产品名称
    Anti-GPCR GPR15抗体
    参阅全部 GPCR GPR15 一抗
  • 描述
    兔多克隆抗体to GPCR GPR15
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Chimpanzee, Macaque monkey
  • 免疫原

    Synthetic peptide:

    YYATSPNSDIRETHSH

    , corresponding to N terminal amino acids 13-28 of Human GPCR GPR15.

  • 阳性对照
    • Jurkat, A549 or K562 cell lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab16445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.25 - 1 µg/ml. Predicted molecular weight: 40 kDa.Can be blocked with GPCR GPR15 peptide (ab6101). Detects a band of approximately 50 kDa in human heart tissue lysate.

靶标

实验方案

Anti-GPCR GPR15 antibody (ab16445)参考文献

ab16445 has not yet been referenced specifically in any publications.

Product Wall

Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH-3T3 cell line)
Gel Running Conditions
Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount
5 µg
Treatment
Cells were mixed in Rippa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification
NIH-3T3 cell line
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

提交于 Oct 15 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cytospined HEK293 human cells)
Permeabilization
No
Specification
Cytospined HEK293 human cells
Fixative
Any
Username

Dr. Francois Daubeuf

Verified customer

提交于 Oct 13 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1, A549, HEK293, CEMx-174)
Gel Running Conditions
Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount
5 µg
Treatment
Cells were mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification
THP-1, A549, HEK293, CEMx-174
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

提交于 Oct 09 2015

Application
Flow Cytometry
Fixation
None, cells were used fresh
Permeabilization
No
Sample
Mouse Cell (Cell from bone marrow)
Specification
Cell from bone marrow
Gating Strategy
Alive cells (DAPI-), blood cells excluded
Preparation
Cell harvesting/tissue preparation method: Bone marrow from Femur was extracted with PBS-EDTA 3mM, 2 hours before flow cytometry analysis
Sample buffer: PBS-EDTA 3mM
Username

Dr. Francois Daubeuf

Verified customer

提交于 Feb 27 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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