兔多克隆抗体to GPCR GPR119
, corresponding to internal sequence amino acids 201-230 of Human GPCR GPR119
WB: extracts from K562 cells
ICC/IF: MCF-7 cells
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Predicted molecular weight: 37 kDa.
1/500 - 1/1000.
Receptor for the endogenous fatty-acid ethanolamide oleoylethanolamide (OEA) and lysophosphatidylcholine (LPC). Functions as a glucose-dependent insulinotropic receptor. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Seems to act through a G(s) mediated pathway.
Predominantly expressed in the pancreas, especially in the islets.
Belongs to the G-protein coupled receptor 1 family.
Information by UniProt
G protein coupled receptor 119 antibody
G protein coupled receptor 2 antibody
G-protein coupled receptor 119 antibody
Anti-GPCR GPR119 antibody 图像
Western blot - GPCR GPR119 antibody (ab75312)
All lanes : Anti-GPCR GPR119 antibody (ab75312) at 1/500 dilution Lane 1 : extracts from K562 cells Lane 2 : extracts from K562 cells with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 37 kDa Observed band size : 37 kDa Additional bands at : 28 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - GPCR GPR119 antibody (ab75312)
Left panel: immunofluorescence analysis of MCF-7 cells, using 1/500 ab75312. Right panel: same, after pre-incubation with the immunizing peptide.
Anti-GPCR GPR119 antibody (ab75312)参考文献
This product has been referenced in:
et al. Characterization of duodenal expression and localization of fatty acid-sensing receptors in humans: relationships with body mass index. Am J Physiol Gastrointest Liver Physiol 307:G958-67 (2014).
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