Anti-GPCR GPR119抗体(ab75312)
Key features and details
- Rabbit polyclonal to GPCR GPR119
- Suitable for: ELISA, IHC-Fr, IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-GPCR GPR119抗体
参阅全部 GPCR GPR119 一抗 -
描述
兔多克隆抗体to GPCR GPR119 -
宿主
Rabbit -
经测试应用
适用于: ELISA, IHC-Fr, IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide:
KMEHAGAMAGGYRSPRTPSDFKALRTVSVL
, corresponding to internal sequence amino acids 201-230 of Human GPCR GPR119 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab75312于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
|
IHC-P | (1) |
Use at an assay dependent concentration.
|
WB |
1/500 - 1/1000. Predicted molecular weight: 37 kDa.
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|
ICC/IF |
1/500 - 1/1000.
|
说明 |
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ELISA
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 37 kDa. |
ICC/IF
1/500 - 1/1000. |
靶标
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功能
Receptor for the endogenous fatty-acid ethanolamide oleoylethanolamide (OEA) and lysophosphatidylcholine (LPC). Functions as a glucose-dependent insulinotropic receptor. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Seems to act through a G(s) mediated pathway. -
组织特异性
Predominantly expressed in the pancreas, especially in the islets. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 139760 Human
- Entrez Gene: 236781 Mouse
- Omim: 300513 Human
- SwissProt: Q8TDV5 Human
- SwissProt: Q7TQP3 Mouse
- Unigene: 496762 Human
- Unigene: 269129 Mouse
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别名
- G protein coupled receptor 119 antibody
- G protein coupled receptor 2 antibody
- G-protein coupled receptor 119 antibody
see all
图片
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All lanes : Anti-GPCR GPR119 antibody (ab75312) at 1/500 dilution
Lane 1 : extracts from K562 cells
Lane 2 : extracts from K562 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
Observed band size: 37 kDa
Additional bands at: 28 kDa, 60 kDa. We are unsure as to the identity of these extra bands. -
Left panel: immunofluorescence analysis of MCF-7 cells, using 1/500 ab75312. Right panel: same, after pre-incubation with the immunizing peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab75312 被引用在 2 文献中.
- Markovics A et al. GPR119 Is a Potent Regulator of Human Sebocyte Biology. J Invest Dermatol 140:1909-1918.e8 (2020). PubMed: 32142797
- Little TJ et al. Characterization of duodenal expression and localization of fatty acid-sensing receptors in humans: relationships with body mass index. Am J Physiol Gastrointest Liver Physiol 307:G958-67 (2014). PubMed: 25258406