Anti-GPCR GPR101抗体(ab75241)

概述

  • 产品名称Anti-GPCR GPR101抗体
    参阅全部 GPCR GPR101 一抗
  • 描述
    兔多克隆抗体to GPCR GPR101
  • 经测试应用适用于: IHC-P, WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthesized peptide derived from C-terminal region of human GPCR GPR101.

  • 阳性对照
    • Extracts from COS-7 cells; HeLa cells.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab75241 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 57 kDa.
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

靶标

Anti-GPCR GPR101 antibody 图像

  • All lanes : Anti-GPCR GPR101 antibody (ab75241) at 1/500 dilution

    Lane 1 : extracts from COS-7 cells
    Lane 2 : extracts from COS-7 cells with immunizing peptide at 5 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 57 kDa
    Observed band size : 57 kDa
  • Left panel: immunofluorescence analysis of HeLa cells with 1/500 ab75241. Right panel: same, after preincubation with the immunizing peptide.
  • IHC image of ab75241 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75241, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-GPCR GPR101 antibody (ab75241)参考文献

ab75241 has not yet been referenced specifically in any publications.

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