概述

  • 产品名称Anti-GPBB抗体[17B6]
    参阅全部 GPBB 一抗
  • 描述
    小鼠单克隆抗体[17B6] to GPBB
  • 特异性This product reacts with BB and MM isoenzyme.
  • 经测试应用适用于: ICC/IF, WB, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length protein (Human).

  • 常规说明Concentration varies from lot to lot and can be provided on request.

性能

应用

Our Abpromise guarantee covers the use of ab880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
AP Use at an assay dependent dilution.
WB Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent dilution. Can be used as Capture antibody. Can be paired for ELISA with Mouse monoclonal [10H5] to GPBB.

靶标

  • 功能Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.
  • 序列相似性Belongs to the glycogen phosphorylase family.
  • 翻译后修饰Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Brain glycogen phosphorylase antibody
    • Glycogen phosphorylase B antibody
    • Glycogen phosphorylase brain form antibody
    • Glycogen Phosphorylase Isoenzyme BB antibody
    • Glycogen phosphorylase, brain form antibody
    • MGC9213 antibody
    • Phosphorylase glycogen brain antibody
    • PYGB antibody
    • PYGB_HUMAN antibody
    see all

Anti-GPBB antibody [17B6] 图像

  • ICC/IF image of ab880 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab880, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-GPBB antibody [17B6] (ab880)参考文献

ab880 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"