产品名称 Anti-GPAA1抗体 参阅全部 GPAA1 一抗
WB, IHC-P more details
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
A region within synthetic peptide: LGSLFLWREL QEAPLSLAEG WQLFLAALAQ GVLEHHTYGA LLFPLLSLGL, corresponding to amino acids 558-607 of Human GPAA1
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
纯度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Detects a band of approximately >70 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
功能 Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate.
组织特异性 Ubiquitously expressed in fetal and adult tissues. Expressed at higher levels in fetal tissues than adult tissues.
通路 Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
细胞定位 Endoplasmic reticulum membrane.
Information by UniProt
Anchor attachment protein 1 (Gaa1p, yeast) homolog antibody
GAA1 protein homolog antibody
Anti-GPAA1 antibody 图像
Western blot - GPAA1 antibody (ab66032)
Anti-GPAA1 antibody (ab66032) at 0.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk / PBS buffer Secondary HRP conjugated anti-rabbit IgG at 50,000 - 100,000 dilution Predicted band size : 68 kDa Observed band size : >70 kDa ( why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-GPAA1 antibody(ab66032)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GPAA1 with ab66032 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
Anti-GPAA1 antibody (ab66032)参考文献
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"