概述

  • 产品名称Anti-GPAA1抗体
    参阅全部 GPAA1 一抗
  • 描述
    兔多克隆抗体to GPAA1
  • 经测试应用适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    A region within synthetic peptide: LGSLFLWREL QEAPLSLAEG WQLFLAALAQ GVLEHHTYGA LLFPLLSLGL, corresponding to amino acids 558-607 of Human GPAA1

  • 阳性对照
    • HepG2 cell lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately >70 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 功能Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate.
  • 组织特异性Ubiquitously expressed in fetal and adult tissues. Expressed at higher levels in fetal tissues than adult tissues.
  • 通路Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
  • 细胞定位Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Anchor attachment protein 1 (Gaa1p, yeast) homolog antibody
    • GAA1 antibody
    • GAA1 protein homolog antibody
    • Glycophosphatidylinositol anchor attachment 1 antibody
    • Glycosylphosphatidylinositol anchor attachment 1 protein antibody
    • Glycosylphosphatidylinositol anchor attachment protein 1 homolog (yeast) antibody
    • GPAA 1 antibody
    • GPAA1 antibody
    • GPAA1_HUMAN antibody
    • GPAA1P anchor attachment protein 1 homolog antibody
    • GPI anchor attachment protein 1 antibody
    • GPI transamidase subunit antibody
    • hGAA1 antibody
    see all

Anti-GPAA1 antibody 图像

  • Anti-GPAA1 antibody (ab66032) at 0.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk / PBS buffer

    Secondary
    HRP conjugated anti-rabbit IgG at 50,000 - 100,000 dilution

    Predicted band size : 68 kDa
    Observed band size : >70 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GPAA1 with ab66032 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-GPAA1 antibody (ab66032)参考文献

ab66032 has not yet been referenced specifically in any publications.

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