Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
A region within synthetic peptide: LGSLFLWREL QEAPLSLAEG WQLFLAALAQ GVLEHHTYGA LLFPLLSLGL, corresponding to amino acids 558-607 of Human GPAA1
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.5 µg/ml. Detects a band of approximately >70 kDa (predicted molecular weight: 68 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 4 - 8 µg/ml.
Essential for GPI-anchoring of precursor proteins but not for GPI synthesis. Acts before or during formation of the carbonyl intermediate.
Ubiquitously expressed in fetal and adult tissues. Expressed at higher levels in fetal tissues than adult tissues.
Glycolipid biosynthesis; glycosylphosphatidylinositol-anchor biosynthesis.
Endoplasmic reticulum membrane.
Information by UniProt
Anchor attachment protein 1 (Gaa1p, yeast) homolog antibody
GAA1 protein homolog antibody
Anti-GPAA1 antibody 图像
Western blot - GPAA1 antibody (ab66032)
Anti-GPAA1 antibody (ab66032) at 0.5 µg/ml + HepG2 cell lysate at 10 µg with 5% skim milk / PBS buffer
HRP conjugated anti-rabbit IgG at 50,000 - 100,000 dilution
Predicted band size :
Observed band size :
>70 kDa (
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-GPAA1 antibody(ab66032)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling GPAA1 with ab66032 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
Anti-GPAA1 antibody (ab66032)参考文献
has not yet been referenced specifically in any publications.
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