Anti-GP210抗体(ab15601)

概述

  • 产品名称Anti-GP210抗体
    参阅全部 GP210 一抗
  • 描述
    兔多克隆抗体to GP210
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human, Monkey
    预测可用于: all Mammals
  • 免疫原

    Fusion protein (Rat) (C terminal).

  • 阳性对照
    • rat liver
  • 常规说明Storage in frost-free freezers is not recommended.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent dilution.
WB 1/1000. Predicted molecular weight: 210 kDa.

靶标

  • 功能Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity.
  • 组织特异性Ubiquitous expression, with highest levels in lung, liver, pancreas, testis, and ovary, intermediate levels in brain, kidney, and spleen, and lowest levels in heart and skeletal muscle.
  • 序列相似性Belongs to the NUP210 family.
  • 翻译后修饰N-glycosylated, but not all potential glycosylation sites may be used. Contains high-mannose type oligosaccharides.
    Phosphorylated at Ser-1881 in mitosis specifically; not phosphorylated in interphase.
  • 细胞定位Nucleus > nuclear pore complex. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ22389 antibody
    • GP 210 antibody
    • KIAA0906 antibody
    • Nuclear envelope pore membrane protein POM 210 antibody
    • Nuclear pore membrane glycoprotein 210 antibody
    • Nuclear pore protein gp210 antibody
    • Nucleoporin 210 antibody
    • Nucleoporin 210kDa antibody
    • Nucleoporin Nup210 antibody
    • Nucleoporin210 antibody
    • NUP 210 antibody
    • Nup210 antibody
    • PO210_HUMAN antibody
    • POM 210 antibody
    • POM210 antibody
    • Pore membrane protein of 210 kDa antibody
    see all

Anti-GP210 antibody 图像



  • Predicted band size : 210 kDa
  • HeLa cells grown on coverslips were washed twice with PBS and permeabilized with 0.2% Triton X-100 in PBS for 2 min at room temperature.  Following 2 PBS washes, the coverslips were fixed with 3.7% formaldehyde and subsequently blocked in 2% skim milk powder in PBS-T (PBS containing 0.1% Tween 20) for 2 hrs.  For detection of gp210, ab15601 was diluted 1:500-1:1000 in 2% skim milk powder in PBS-T and incubated on the coverslips for 1 hour at RT.  Following incubation with primary antibodies, coverslips were washed three times with PBS-T over a period of 45 min.  Antibody binding was detected with donkey anti-rabbit antibodies conjugated to Cy3 at a dilution of 1:2500.

Anti-GP210 antibody (ab15601)参考文献

ab15601 has not yet been referenced specifically in any publications.

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