概述

  • 产品名称Anti-GP210抗体
    参阅全部 GP210 一抗
  • 描述
    兔多克隆抗体to GP210
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Rat, Xenopus laevis
    预测可用于: all Mammals
  • 免疫原

    Purified bacterial N-terminal fusion protein

  • 常规说明Storage in frost-free freezers is not recommended.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.1% Sodium azide
    Constituent: Whole serum
  • 纯度Whole antiserum
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000. Predicted molecular weight: 210 kDa.
ICC/IF Use at an assay dependent dilution.

靶标

  • 功能Nucleoporin essential for nuclear pore assembly and fusion, nuclear pore spacing, as well as structural integrity.
  • 组织特异性Ubiquitous expression, with highest levels in lung, liver, pancreas, testis, and ovary, intermediate levels in brain, kidney, and spleen, and lowest levels in heart and skeletal muscle.
  • 序列相似性Belongs to the NUP210 family.
  • 翻译后修饰N-glycosylated, but not all potential glycosylation sites may be used. Contains high-mannose type oligosaccharides.
    Phosphorylated at Ser-1881 in mitosis specifically; not phosphorylated in interphase.
  • 细胞定位Nucleus > nuclear pore complex. Nucleus membrane. Endoplasmic reticulum membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ22389 antibody
    • GP 210 antibody
    • KIAA0906 antibody
    • Nuclear envelope pore membrane protein POM 210 antibody
    • Nuclear pore membrane glycoprotein 210 antibody
    • Nuclear pore protein gp210 antibody
    • Nucleoporin 210 antibody
    • Nucleoporin 210kDa antibody
    • Nucleoporin Nup210 antibody
    • Nucleoporin210 antibody
    • NUP 210 antibody
    • Nup210 antibody
    • PO210_HUMAN antibody
    • POM 210 antibody
    • POM210 antibody
    • Pore membrane protein of 210 kDa antibody
    see all

Anti-GP210 antibody 图像

  • HeLa cells were permeabilised in PBS Triton X-100 (0.2%), fixed in 3.7% formaldehyde and blocked in 2% milk in PBS-T. The slides were incubated with 1/1000 dilution of ab15600 for 1 hour at room temperature. The slides were washed 3 times prior to incubation with the secondary antibody. ab15600 clearly stains the nuclear envelope (red). The DNA is stained with DAPI (blue).

Anti-GP210 antibody (ab15600)参考文献

This product has been referenced in:
  • Fernández-Medarde A  et al. Laser microdissection and microarray analysis of the hippocampus of Ras-GRF1 knockout mice reveals gene expression changes affecting signal transduction pathways related to memory and learning. Neuroscience 146:272-85 (2007). Read more (PubMed: 17321057) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
Sample African Green Monkey Cell (Kidney epithelial cell (Vero))
Specification Kidney epithelial cell (Vero)
Permeabilization Yes - 0.2% triton x-100
Fixative Paraformaldehyde
Username

Eric Alonas

Verified customer

提交于 Nov 18 2014

Thank you very much for contacting us with your question.

We only have full size vials of all of our products, so unfortunately we do not have any smalltrialvials to send out. Do you have the Arabidopsis amino acid sequence for GP210? I could...

Read More

Thank you for the details that you have provided and for your patience. Did you also try this antibody in Western blotting? I saw a note from a colleague who spoke to you and both IHC and WB are mentioned as applications that you have tried. If yes, ca...

Read More

Thank you for contacting us. Please find links to the forms I discussed with you (one for IHC, one for Western blot). Once you have submitted these forms, we will take a look at your protocol and try to make any suggestions we think will help impro...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"