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Our Abpromise guarantee covers the use of ab15600 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 210 kDa.|
|ICC/IF||Use at an assay dependent dilution.|
HeLa cells were permeabilised in PBS Triton X-100 (0.2%), fixed in 3.7% formaldehyde and blocked in 2% milk in PBS-T. The slides were incubated with 1/1000 dilution of ab15600 for 1 hour at room temperature. The slides were washed 3 times prior to incubation with the secondary antibody. ab15600 clearly stains the nuclear envelope (red). The DNA is stained with DAPI (blue).