重组
RabMAb

Anti-GOSR1抗体[EP1768Y] (ab53288)

概述

  • 产品名称
    Anti-GOSR1抗体[EP1768Y]
    参阅全部 GOSR1 一抗
  • 描述
    兔单克隆抗体[EP1768Y] to GOSR1
  • 特异性
    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • 经测试应用
    适用于: ICC/IF, WB, ICC, Flow Cyt, IHC-Pmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    corresponding to Human GOSR1 (N terminal).

  • 阳性对照
    • SKBR-3 cell lysate 3T3 cell lysate HeLa cells Human stomach carcinoma
  • 常规说明

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab53288 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/150.

For unpurified use at 1/50 - 1/100. 

WB 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

For unpurified use at 1/250 - 1/500. 

ICC 1/50 - 1/100.
Flow Cyt 1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

For unpurified use at 1/30.

IHC-P 1/100.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 相关性
      GOSR1 is a trafficking membrane protein which transports proteins among the endoplasmic reticulum and the Golgi and between Golgi compartments; it is considered an essential component of the Golgi SNAP receptor (SNARE) complex.
    • 细胞定位
      Cell Membrane, Endoplasmic reticulum and Golgi Apparatus
    • 数据库链接
    • 别名
      • 28 kDa cis Golgi SNARE p28 antibody
      • 28 kDa Golgi SNARE protein antibody
      • cis-golgi SNARE antibody
      • golgi integral membrane protein 2 antibody
      • Golgi SNAP receptor complex member 1 antibody
      • Golgi SNARE 28 kDa antibody
      • GOLIM2 antibody
      • GOS28 antibody
      • GOS28/P28 antibody
      • GOSR1 antibody
      • GS25 antibody
      • Gs28 antibody
      • P28 antibody
      see all

    图片

    • All lanes : Anti-GOSR1 antibody [EP1768Y] (ab53288) at 1/500 dilution (unpurified)

      Lane 1 : SKBR-3 cell lysate
      Lane 2 : 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 29 kDa
      Observed band size : 29 kDa
    • HeLa cells stained with unpurified ab53288 at 1/50 - 1/100 dilution

    • Human stomach carcinoma staining with unpurified ab53288 at 1/100 - 1/250 dilution

    文献

    ab53288 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (HEK 293T cell transfected with pGOSR1-EGFP)
    Loading amount
    20 µg
    Specification
    HEK 293T cell transfected with pGOSR1-EGFP
    Gel Running Conditions
    Reduced Denaturing (4-15)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 25 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册