重组Anti-GOSR1/GS28抗体[EP1768Y] (ab53288)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1768Y] to GOSR1/GS28
- Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-GOSR1/GS28抗体[EP1768Y]
参阅全部 GOSR1/GS28 一抗 -
描述
兔单克隆抗体[EP1768Y] to GOSR1/GS28 -
宿主
Rabbit -
特异性
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. -
经测试应用
适用于: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details
不适用于: IP -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human GOSR1/GS28 (N terminal). The exact sequence is proprietary.
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阳性对照
- SKBR-3 cell lysate 3T3 cell lysate HeLa cells Human stomach carcinoma
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP1768Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53288于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
1/120.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/30. |
|
ICC/IF |
1/150.
For unpurified use at 1/50 - 1/100. |
|
WB | (1) |
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
For unpurified use at 1/250 - 1/500. |
IHC-P |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
说明 |
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Flow Cyt (Intra)
1/120. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For unpurified use at 1/30. |
ICC/IF
1/150. For unpurified use at 1/50 - 1/100. |
WB
1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa). For unpurified use at 1/250 - 1/500. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. |
靶标
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相关性
GOSR1/GS28 is a trafficking membrane protein which transports proteins among the endoplasmic reticulum and the Golgi and between Golgi compartments; it is considered an essential component of the Golgi SNAP receptor (SNARE) complex. -
细胞定位
Cell Membrane, Endoplasmic reticulum and Golgi Apparatus -
数据库链接
- Entrez Gene: 9527 Human
- Entrez Gene: 53334 Mouse
- Entrez Gene: 94189 Rat
- Omim: 604026 Human
- SwissProt: O95249 Human
- SwissProt: O88630 Mouse
- SwissProt: Q62931 Rat
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别名
- 28 kDa cis Golgi SNARE p28 antibody
- 28 kDa Golgi SNARE protein antibody
- cis-golgi SNARE antibody
see all
图片
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling GOSR1/GS28 with purified ab53288 at 1/120 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry/ Immunofluorescence analysis of NIH/3T3 (Mouse embryonic fibroblast) cells labeling GOSR1/GS28 with Purified ab53288 at 1:150 dilution. Cells were fixed in 100% Methanol. Cells were counterstained with None. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling GOSR1/GS28 with Purified ab53288 at 1:100 dilution (12.3 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
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All lanes : Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288) at 1/1000 dilution (purified)
Lane 1 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
Lane 3 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 6 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lane 7 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDaBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-GOSR1/GS28 antibody [EP1768Y] (ab53288) at 1/500 dilution (unpurified)
Lane 1 : SKBR-3 cell lysate
Lane 2 : 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa -
HeLa cells stained with unpurified ab53288 at 1/50 - 1/100 dilution
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Human stomach carcinoma staining with unpurified ab53288 at 1/100 - 1/250 dilution
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab53288 被引用在 1 文献中.
- Hayashi Y et al. Complex formation of sphingomyelin synthase 1 with glucosylceramide synthase increases sphingomyelin and decreases glucosylceramide levels. J Biol Chem 293:17505-17522 (2018). PubMed: 30242129