Anti-GOLPH3/MIDAS抗体(ab91492)
Key features and details
- Rabbit polyclonal to GOLPH3/MIDAS
- Suitable for: ICC, WB
- Reacts with: Rat
- Isotype: IgG
概述
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产品名称
Anti-GOLPH3/MIDAS抗体
参阅全部 GOLPH3/MIDAS 一抗 -
描述
兔多克隆抗体to GOLPH3/MIDAS -
宿主
Rabbit -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Rat -
免疫原
Synthetic peptide corresponding to Human GOLPH3/MIDAS (N terminal).
Database link: AAH12123 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab91492于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC |
Use at an assay dependent concentration.
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WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa.
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说明 |
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ICC
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 34 kDa. |
靶标
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功能
Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene. -
组织特异性
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers. -
序列相似性
Belongs to the GOLPH3/VPS74 family. -
翻译后修饰
Phosphorylated. -
细胞定位
Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space. - Information by UniProt
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数据库链接
- Entrez Gene: 78961 Rat
- SwissProt: Q9ERE4 Rat
- Unigene: 64519 Rat
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别名
- Coat protein antibody
- Coat protein GPP34 antibody
- FLJ90675 antibody
see all
图片
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Lane 1 : Anti-GOLPH3/MIDAS antibody (ab91492) at 0.5 µg/ml
Lane 2 : Anti-GOLPH3/MIDAS antibody (ab91492) at 1 µg/ml
All lanes : rat lung tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 34 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa, 42 kDa, 50 kDa. We are unsure as to the identity of these extra bands. -
ab91492 staining GOLPH3/MIDAS in human K562 cells by Immunocytochemistry/ Immunofluorescence.Cells were fixed in formaldehyde, blocked with 2% serum for 1 hour at 22°C and then incubated with ab91492 at a 1/150 dilution for 1 hour at 22°C. The secondary used was a DyLight 594 conjugated donkey anti-rabbit polyclonal used at a 1/200 dilution.Blue is nucleus staining by DAPI, red is GOLPH3/MIDAS.
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ab91492, staining GOLPH3/MIDAS (red) in HCV-infected Huh 7.5.1 cells, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in 3% paraformaldehyde, permeabilized and blocked in antibody-binding buffer for 2 hours at 4°C. Samples were incubated with primary antibody and a fluorescence-conjugated donkey anti-rabbit IgG was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). -
ab91492, staining GOLPH3/MIDAS in bovine aortic endothelial cells, by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton X-100/PBS for 15 min at RT and blocked with 5% donkey serum/0.3% BSA/PBS for 30 min at RT. Samples were incubated with primary antibody at 1/100 dilution overnight at 4°C. An AlexaFlour®568-conjugated anti-rabbit IgG (1/500) was used as the secondary antibody. -
Immunofluorescence of GOLPH3/MIDAS in Rat Lung cells using ab91492 at 20 ug/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab91492 被引用在 9 文献中.
- Li Y et al. Suppressing MDSC Infiltration in Tumor Microenvironment Serves as an Option for Treating Ovarian Cancer Metastasis. Int J Biol Sci 18:3697-3713 (2022). PubMed: 35813475
- Zhao C et al. GOLPH3 Promotes Angiogenesis of Lung Adenocarcinoma by Regulating the Wnt/ß-Catenin Signaling Pathway. Onco Targets Ther 13:6265-6277 (2020). PubMed: 32636646
- Peñalver-González B et al. Golgi Oncoprotein GOLPH3 Gene Expression Is Regulated by Functional E2F and CREB/ATF Promoter Elements. Genes (Basel) 10:N/A (2019). PubMed: 30934642
- Song Q et al. ATF-3/miR-590/GOLPH3 signaling pathway regulates proliferation of breast cancer. BMC Cancer 18:255 (2018). PubMed: 29534690
- Tang S et al. Expression of GOLPH3 in patients with non-small cell lung cancer and xenografts models. Oncol Lett 15:7555-7562 (2018). PubMed: 29849795
- Tang S et al. GOLPH3: a novel biomarker that correlates with poor survival and resistance to chemotherapy in breast cancer. Oncotarget 8:105155-105169 (2017). PubMed: 29285241
- Zhang L et al. Golgi phosphoprotein 3 expression predicts poor prognosis in patients with prostate cancer undergoing radical prostatectomy. Mol Med Rep 12:1298-304 (2015). PubMed: 25760033
- Sangwung P et al. Proteomic identification of S-nitrosylated Golgi proteins: new insights into endothelial cell regulation by eNOS-derived NO. PLoS One 7:e31564 (2012). ICC/IF ; Cow . PubMed: 22363674
- Bishé B et al. Role of Phosphatidylinositol 4-Phosphate (PI4P) and Its Binding Protein GOLPH3 in Hepatitis C Virus Secretion. J Biol Chem 287:27637-47 (2012). ICC/IF ; Human . PubMed: 22745132