概述

  • 产品名称Anti-GOLPH3抗体
    参阅全部 GOLPH3 一抗
  • 描述
    小鼠多克隆抗体to GOLPH3
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length GOLPH3 protein (Human) (NP_071413)

  • 阳性对照
    • GOLPH3 transfected 293T cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab69171 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 34 kDa).

靶标

  • 功能Involved in modulation of mTOR signaling. Involved in the regulation of mitochondrial lipids, leading to increase of mitochondrial mass. Potential oncogene.
  • 组织特异性Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
  • 序列相似性Belongs to the GOLPH3/VPS74 family.
  • 翻译后修饰Phosphorylated.
  • 细胞定位Cell membrane. Endosome. Cytoplasm. Golgi apparatus > Golgi stack membrane. Mitochondrion intermembrane space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Coat protein antibody
    • Coat protein GPP34 antibody
    • FLJ90675 antibody
    • Golgi associated protein antibody
    • Golgi peripheral membrane protein 1, 34 kDa antibody
    • Golgi phosphoprotein 3 (coat protein) antibody
    • Golgi phosphoprotein 3 antibody
    • Golgi protein antibody
    • GOLP3_HUMAN antibody
    • Golph3 antibody
    • GOPP1 antibody
    • GPP34 antibody
    • MIDAS antibody
    • Mitochondrial DNA absence factor antibody
    see all

Anti-GOLPH3 antibody 图像

  • All lanes : Anti-GOLPH3 antibody (ab69171) at 1/500 dilution

    Lane 1 : GOLPH3 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 34 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ab69171 staining GOLPH3 in human KG1a cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, blocked with 2% serum for 1 hour at 22°C and then incubated with ab69171 at a 1/100 dilution for 1 hour at 22°C. The secondary used was a DyLight 488 conjugated donkey anti-mouse polyclonal used at a 1/200 dilution.

    Blue is nucleus staining by DAPI, green is GOLPH3.

    See Abreview

Anti-GOLPH3 antibody (ab69171)参考文献

This product has been referenced in:
  • Li H  et al. GOLPH3 overexpression correlates with tumor progression and poor prognosis in patients with clinically N0 oral tongue cancer. J Transl Med 10:168 (2012). WB . Read more (PubMed: 22905766) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (KG1a)
Specification KG1a
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Mar 14 2012

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