概述

  • 产品名称Anti-GOLPH2抗体
    参阅全部 GOLPH2 一抗
  • 描述
    兔多克隆抗体to GOLPH2
  • 特异性ab22209 reacts with Golgi phosphoprotein 2 / GOLPH2.
  • 经测试应用适用于: IHC-P, ELISA, ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide:

    KNEFQGELEK QREQL

    , corresponding to amino acids 67-81 of Human GOLPH2.

性能

应用

Our Abpromise guarantee covers the use of ab22209 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use at an assay dependent concentration.
ELISA 1/100 - 1/1000.
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

靶标

  • 功能Unknown. Cellular response protein to viral infection.
  • 组织特异性Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte-derived syncitial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes.
  • 序列相似性Belongs to the GOLM1/CASC4 family.
  • 翻译后修饰Glycosylated.
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位Golgi apparatus > cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bA379P1.3 antibody
    • C9orf155 antibody
    • Chromosome 9 open reading frame 155 antibody
    • Golgi membrane protein 1 antibody
    • Golgi membrane protein GP73 antibody
    • Golgi phosphoprotein 2 antibody
    • Golgi protein 73 kD antibody
    • Golgi protein 73kD antibody
    • GOLM 1 antibody
    • GOLM1 antibody
    • GOLM1_HUMAN antibody
    • GOLPH 2 antibody
    • GOLPH2 antibody
    • GP 73 antibody
    • GP73 antibody
    • PSEC0257 antibody
    see all

Anti-GOLPH2 antibody 图像

  • ICC/IF image of ab22209 stained Hek293 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22209, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab22209 (4µg/ml) staining gGOLPH2n human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic within the colonic epithelium.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • All lanes : Anti-GOLPH2 antibody (ab22209) at 1 µg/ml

    Lane 1 : Human colon tissue lysate - total protein (ab30051)
    Lane 2 : Prostate Tumor Lysate Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 27 kDa,42 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

Anti-GOLPH2 antibody (ab22209)参考文献

This product has been referenced in:
  • Riener MO  et al. Golgi phosphoprotein 2 (GOLPH2) expression in liver tumors and its value as a serum marker in hepatocellular carcinomas. Hepatology 49:1602-9 (2009). ELISA ; Human . Read more (PubMed: 19291786) »
  • Kristiansen G  et al. GOLPH2 protein expression as a novel tissue biomarker for prostate cancer: implications for tissue-based diagnostics. Br J Cancer 99:939-48 (2008). IHC-P ; Human . Read more (PubMed: 18781151) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry.

Please follow this link to the datasheet of ab22209 and all the information available about this product:

http://www.abcam.com/index.html?datasheet=22209 (or use the following: http://www.abcam.com/index....

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"