The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 45 kDa.
Use a concentration of 3.75 µg/ml.
功能Unknown. Cellular response protein to viral infection.
组织特异性Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocyte-derived syncitial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes.
序列相似性Belongs to the GOLM1/CASC4 family.
翻译后修饰Glycosylated. Phosphorylation sites are present in the extracelllular medium.
细胞定位Golgi apparatus > cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.
ab110008, at 3.75 µg/ml, staining GOLPH2 in Human uterus tissue by immunohistochemistry.
Western blot - GOLPH2 antibody (ab110008)
Lane 1 : Anti-GOLPH2 antibody (ab110008) at 1 µg/ml Lane 2 : Anti-GOLPH2 antibody (ab110008) at 1 mg/ml Lane 3 : anti-Myc tag at 1/1000 dilution
Lane 1 : HEK293 lysate (in RIPA buffer) overexpressing Human GOLM1 with C-terminal MYC tag at 10 µg Lane 2 : Mock-transfected HEK293 Lane 3 : HEK293 lysate (in RIPA buffer) overexpressing Human GOLM1 with C-terminal MYC tag at 10 µg
Predicted band size : 45 kDa
Anti-GOLPH2 antibody (ab110008)参考文献
has not yet been referenced specifically in any publications.