Anti-Golgin subfamily A member 4抗体(ab122250)

概述

  • 产品名称
    Anti-Golgin subfamily A member 4抗体
  • 描述
    兔多克隆抗体to Golgin subfamily A member 4
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen, corresponding to amino acids 1482-1584 of Human Golgin subfamily A member 4 (UniProt: Q13439).

  • 阳性对照
    • Human stomach tissue

性能

应用

Our Abpromise guarantee covers the use of ab122250 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    May play role in delivery of transport vesicles containing GPI-linked proteins from the trans-Golgi network through its interaction with MACF1.
  • 序列相似性
    Contains 1 GRIP domain.
  • 结构域
    Extended rod-like protein with coiled-coil domains.
  • 细胞定位
    Cytoplasm. Golgi apparatus membrane. Golgi apparatus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 256 kDa golgin antibody
    • 72.1 protein antibody
    • GCP2 antibody
    • GOGA4_HUMAN antibody
    • GOLG antibody
    • GOLGA4 antibody
    • golgi autoantigen, golgin subfamily a, 4 antibody
    • Golgin subfamily A member 4 antibody
    • golgin-240 antibody
    • Golgin-245 antibody
    • MU-RMS-40.18 antibody
    • OTTHUMP00000161363 antibody
    • OTTHUMP00000208897 antibody
    • OTTHUMP00000209805 antibody
    • p230 antibody
    • Protein 72.1 antibody
    • Trans-Golgi p230 antibody
    see all

图片

  • Immunofluorescent staining of human cell line U-2 OS shows positivity in cytoplasm & vesicles.
  • ab122250, at 1/1200, staining Golgin subfamily A member 4 in paraffin embedded Human stomach tissue by Immunohistochemistry.

文献

ab122250 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HepG2 cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
HepG2 cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Dec 21 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Dec 21 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
25 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Dec 12 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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