概述

  • 产品名称Anti-Golgin 97抗体
    参阅全部 Golgin 97 一抗
  • 描述
    兔多克隆抗体to Golgin 97
  • 经测试应用适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human Golgin 97.

    (Peptide available as ab34458.)

  • 阳性对照
    • This antibody gave a positive signal when used to probe HepG2 cells (IF) and human breast carcinoma (IHC)
  • 常规说明ab84340 is raised using the same immunogen as ab33701. Batches sold under ab84340 have passed QC testing in ICC/IF and IHC. In our hands, this product did not work in WB. For further information please contact our Customer Services team.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab84340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能Probably involved in maintaining Golgi structure.
  • 序列相似性Contains 1 GRIP domain.
  • 结构域Extended rod-like protein with coiled-coil domains.
  • 细胞定位Golgi apparatus > Golgi stack membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Gap junction protein alpha 4 37kD antibody
    • GOGA1_HUMAN antibody
    • Golga 1 antibody
    • GOLGA1 antibody
    • Golgi autoantigen golgin subfamily a 1 antibody
    • Golgi autoantigen golgin subfamily A member 1 antibody
    • Golgi autoantigen golgin subfamily a1. antibody
    • Golgin A1 antibody
    • Golgin subfamily A member 1 antibody
    • Golgin-97 antibody
    • Golgin97 antibody
    • MGC33154 antibody
    see all

Anti-Golgin 97 antibody 图像

  • ICC/IF image of ab84340 stained human HepG2 cells. The cells were methanol fixed (5 min), permeabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab84340, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
  • IHC image of Golgin 97 staining in human breast carcinoma FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84340, 5µg/ml, for 8 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • ab84340 staining Golgin 97 in murine testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 0.01M sodium citrate. Samples were then blocked using 10%FCS, 0.5% BSA, 1% Triton X-100, 88.5% PBS X1 and then incubated with ab84340 at a 1/100 dilution for 10 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit IgG used at a 1/200 dilution.

    See Abreview

  • ab84340 staining Golgin-97 in MCF7 cells treated with brefeldin A (ab120299), by ICC/IF. Increase in Golgin-97 expression correlates with increased concentration of brefeldin A, as described in literature.
    The cells were incubated at 37øC for 1.5 h in media containing different concentrations of ab120299 (brefeldin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab84340 (5 µg/ml) was performed overnight at 4øC in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-Golgin 97 antibody (ab84340)参考文献

This product has been referenced in:
  • Lee I  et al. Membrane adhesion dictates Golgi stacking and cisternal morphology. Proc Natl Acad Sci U S A 111:1849-54 (2014). Read more (PubMed: 24449908) »
  • Yermakova A  et al. Antibody-mediated inhibition of ricin toxin retrograde transport. MBio 5:e00995 (2014). ICC/IF ; African Green Monkey . Read more (PubMed: 24713323) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (hCMEC/D3 endothelial cell line)
Permeabilization Yes - 0.3% Triton
Specification hCMEC/D3 endothelial cell line
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (testis)
Specification testis
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M sodium citrate
Permeabilization No
Blocking step 10%FCS 0.5% BSA 1% Triton X-100 88.5% PBSX1 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Mr. Yoav Elkis

Verified customer

提交于 Jan 04 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"