Anti-Golgi Complex抗体(ab103439)


  • 产品名称Anti-Golgi Complex抗体
    参阅全部 Golgi Complex 一抗
  • 描述
    兔多克隆抗体to Golgi Complex
  • 经测试应用适用于: WB, IHC-P, IHC-FoFr, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Chicken, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to a region within C terminal amino acids 1150-1179 of Human Golgi Complex.

  • 阳性对照
    • MCF7 cell line and Mouse cerebellum tissue lysates; skeletal muscle tissue. This antibody gave a positive result when used in the following formaldehyde fixed cell line: HepG2.


  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab103439 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域


Our Abpromise guarantee covers the use of ab103439 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 135 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-FoFr Use at an assay dependent concentration.
ICC/IF 1/200.


  • 功能Binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils).
  • 组织特异性Widely expressed. Highest levels in pancreas, skeletal muscle, placenta, heart, testis and ovary. Also found in the kidney, liver, lung and brain.
  • 序列相似性Contains 16 Cys-rich GLG1 repeats.
  • 发展阶段Expressed both in adult and fetal tissues.
  • 翻译后修饰Fucosylation is essential for binding to E-selectin.
    N-glycosylated. Contains sialic acid residues.
  • 细胞定位Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AI593353 antibody
    • AW537898 antibody
    • CFR 1 antibody
    • CFR-1 antibody
    • CFR1 antibody
    • Cys -rich FGF Receptor antibody
    • Cysteine rich fibroblast growth factor receptor antibody
    • Cysteine-rich fibroblast growth factor receptor antibody
    • DKFZp686L08213 antibody
    • E selectin ligand 1 antibody
    • E-selectin ligand 1 antibody
    • ESL 1 antibody
    • ESL-1 antibody
    • ESL1 antibody
    • FLJ23319 antibody
    • FLJ23967 antibody
    • FLJ32797 antibody
    • GLG1 antibody
    • Golgi apparatus protein 1 antibody
    • golgi glycoprotein 1 antibody
    • Golgi sialoglycoprotein MG 160 antibody
    • Golgi sialoglycoprotein MG-160 antibody
    • GSLG1_HUMAN antibody
    • MG 160 antibody
    • MG160 antibody
    • Mouse, homolog of, ESL1 antibody
    • Selel antibody
    • Slectin, endothelial cell, ligand antibody
    see all

Anti-Golgi Complex antibody 图像

  • ICC/IF image of ab103439 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103439 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Golgi Complex antibody (ab103439) at 1/100 dilution + MCF7 cell line lysates at 35 µg

    Predicted band size : 135 kDa
  • Anti-Golgi Complex antibody (ab103439) at 1/100 dilution + Mouse cerebellum tissue lysates at 35 µg

    Predicted band size : 135 kDa
  • ab103439 at 1/50 dilution, staining Golgi Complex in Human skeletal muscle by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
  • ab103439 staining Golgi Complex in murine spinal cord motor neurons by Immunohistochemistry (PFA perfusion fixed frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using 0.2% Triton/PBS, blocked with 2.5% serum for 1 hour at 20°C and then incubated with ab103439 at a 1/500 dilution for 12 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal used at a 1/500 dilution.

    See Abreview

Anti-Golgi Complex antibody (ab103439)参考文献

ab103439 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Sample Chicken Cell (Primary oculomotor culture)
Specification Primary oculomotor culture
Permeabilization Yes - PBS + 0.1% Tween20
Fixative Formaldehyde

Abcam user community

Verified customer

提交于 Oct 27 2014

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (Breast Cancer MDA-MB-231 Cells/ Melanoma MDA-MB-43)
Specification Breast Cancer MDA-MB-231 Cells/ Melanoma MDA-MB-43
Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 50% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Aug 28 2014

Thank you for taking time to contact us and forward an image of your staining. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with...

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Merci de nous avoir contactés.

Nous avons maintenantvalidé la commande. Le produit doit être envoyé de notre stock des Etats-Unis, vous devriez le recevoir mercredi 29 février.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Spinal cord motor neuron)
Specification Spinal cord motor neuron
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization Yes - PBS Triton 0.1%
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 20°C

Dr. Georg Haase

Verified customer

提交于 Feb 24 2012

Code de remise : ********
Date d’expiration : JJ MM AAAA

Je suis ravi que vous souhaitiez participerà notre offre et tester ab103439 en cytométrie en flux et/ou IHC-Fr. Le code de réduction correspondant est *...

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