Synthetic peptide, corresponding to a region within amino acids 1436 and 1498 of GOLGA3 (NP_005886).
293T, A431, H1299 cell lysates for WB and HeLa cells for IF
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 1% BSA, 1X PBS, pH 7
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Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 167 kDa.
1/100 - 1/200.
GOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization.
Cytoplasmic and Golgi Apparatus
GOLGA 3 antibody
Western blot - GOLGA3 antibody (ab96080)
All lanes : Anti-GOLGA3 antibody (ab96080) at 1/1000 dilution Lane 1 : A431 whole cell lysate Lane 2 : H1299 whole cell lysate Lysates/proteins at 30 µg per lane. Predicted band size: 167 kDa 5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - GOLGA3 antibody (ab96080)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96080 at 1/200 dilution. Lower image merged with DNA probe.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"