Anti-GOLGA3抗体(ab96080)
Key features and details
- Rabbit polyclonal to GOLGA3
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-GOLGA3抗体
参阅全部 GOLGA3 一抗 -
描述
兔多克隆抗体to GOLGA3 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide, corresponding to a region within amino acids 1436 and 1498 of GOLGA3 (NP_005886).
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阳性对照
- 293T, A431, H1299 cell lysates for WB and HeLa cells for IF
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96080于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 167 kDa.
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ICC/IF |
1/100 - 1/200.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 167 kDa. |
ICC/IF
1/100 - 1/200. |
靶标
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功能
Golgi auto-antigen; probably involved in maintaining Golgi structure. -
组织特异性
Expressed in all tissues tested. Expressed in liver, testis, lung, heart, salivary gland and kidney. -
结构域
Extended rod-like protein with coiled-coil domains. -
翻译后修饰
Cleaved by caspases in apoptotic cells. -
细胞定位
Cytoplasm. Golgi apparatus, Golgi stack membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 2802 Human
- Omim: 602581 Human
- SwissProt: Q08378 Human
- Unigene: 507333 Human
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别名
- GCP170 antibody
- GOGA3_HUMAN antibody
- GOLGA 3 antibody
see all
图片
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All lanes : Anti-GOLGA3 antibody (ab96080) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 167 kDa
5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, using ab96080 at 1/200 dilution. Lower image merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab96080 被引用在 3 文献中.
- Yue X et al. Tankyrase-1-mediated degradation of Golgin45 regulates glycosyltransferase trafficking and protein glycosylation in Rab2-GTP-dependent manner. Commun Biol 4:1370 (2021). PubMed: 34876695
- Tang CX et al. Golgin-160 and GMAP210 play an important role in U251 cells migration and invasion initiated by GDNF. PLoS One 14:e0211501 (2019). PubMed: 30695072
- Turowec JP et al. An unbiased proteomic screen reveals caspase cleavage is positively and negatively regulated by substrate phosphorylation. Mol Cell Proteomics 13:1184-97 (2014). WB ; Human . PubMed: 24556848