概述

  • 产品名称Anti-GODZ抗体
    参阅全部 GODZ 一抗
  • 描述
    兔多克隆抗体to GODZ
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
    预测可用于: Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Mouse GODZ.

    (Peptide available as ab31882.)

  • 阳性对照
    • Mouse Cerebellum Tissue Lysate

性能

应用

Our Abpromise guarantee covers the use of ab31837 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration. PubMed: 19001095
IHC-P 1/75. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

靶标

  • 功能Palmitoyltransferase with broad specificity. Palmitoylates GABA receptors on their gamma subunit (GABRG1, GABRG2 and GABRG3), which regulates synaptic clustering and/or cell surface stability. Palmitoylates glutamate receptors GRIA1 and GRIA2, which leads to their retention in Golgi.
  • 序列相似性Belongs to the DHHC palmitoyltransferase family.
    Contains 1 DHHC-type zinc finger.
  • 结构域The DHHC domain is required for palmitoyltransferase activity.
  • 翻译后修饰Autopalmitoylated.
  • 细胞定位Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DHHC-3 antibody
    • GABA-A receptor-associated membrane protein 1 antibody
    • Golgi-specific DHHC zinc finger protein antibody
    • Gramp1 antibody
    • Palmitoyltransferase ZDHHC3 antibody
    • Protein DHHC1 antibody
    • ZDHC3 antibody
    • ZDHC3_HUMAN antibody
    • ZDHHC3 antibody
    • Zinc finger DHHC domain-containing protein 3 antibody
    • Zinc finger protein 373 antibody
    • ZNF373 antibody
    see all

Anti-GODZ antibody 图像

  • (Image courtesy of Human Protein Atlas)
    ab31837 detecting GODZ protein in the purkinje cells of normal human cerebellum. Paraffin embedded human cerebral cortex tissue was incubated with ab31837 (1/75 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

  • Anti-GODZ antibody (ab31837) at 1 µg/ml + Cerebellum (Mouse) Tissue Lysate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

Anti-GODZ antibody (ab31837)参考文献

This product has been referenced in:
  • Levy AD  et al. Subcellular Golgi localization of stathmin family proteins is promoted by a specific set of DHHC palmitoyl transferases. Mol Biol Cell 22:1930-42 (2011). Read more (PubMed: 21471001) »
  • Shmueli A  et al. Ndel1 palmitoylation: a new mean to regulate cytoplasmic dynein activity. EMBO J 29:107-19 (2010). WB, ICC/IF ; Human . Read more (PubMed: 19927128) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us.

Here are the links to the Human Protein Atlas as well as to the mouse protein entry in UniProt database:

http://www.proteinatlas.org/ENSG00000163812/cell
http://www.proteinatlas.org/ENSG00000163812
Read More

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

提交于 Sep 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"