山羊anti-Rat IgG H&L (PE) secondary抗体(ab7010)

概述

  • 产品名称
    山羊anti-Rat IgG H&L (PE)二抗
    参阅全部 IgG 二抗
  • 靶标种属
    Rat
  • 经测试应用
    适用于: ICC/IF, IHC-P, IHC-Fr, Immunomicroscopy, Flow Cytmore details
  • 最小交叉反应

    Cow, Horse, Human, Mouse, Rabbit, Sheep more details
  • 免疫原

    Full length native Rat IgG (purified).

  • 偶联物
    Phycoerythrin. Ex: 488nm, Em: 575nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Prepared from monospecific antiserum by immunoaffinity chromatography using rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation
  • 共轭说明
    R-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明


    Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7010 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy 1/50 - 1/200.
Flow Cyt 1/100 - 1/250.

Use 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate.

Goat anti-Rat IgG H&L (PE) secondary antibody (ab7010)参考文献

This product has been referenced in:
  • Feng D  et al. Reduced EBP50 expression levels are correlated with unfavorable clinicopathological features of extrahepatic bile duct carcinoma and promote the proliferation and migration of QBC939 cells. Oncol Lett 13:2758-2764 (2017). IHC-P . Read more (PubMed: 28454463) »
  • Zhang ZH  et al. An integrated lipidomics and metabolomics reveal nephroprotective effect and biochemical mechanism of Rheum officinale in chronic renal failure. Sci Rep 6:22151 (2016). IHC-P . Read more (PubMed: 26903149) »

See all 4 Publications for this product

Product Wall

Thank you for your enquiry and for bringing this matter to my attention. A mistake appears to have occurred on our product data sheet and the value should read ug and not mg. I have updated the product data sheet to reflect this change. I apologise for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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