山羊抗大鼠IgG H&L (DyLight® 488)预吸附二抗(ab98420)
Key features and details
- Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed
- Conjugation: DyLight® 488. Ex: 493nm, Em: 518nm
- Host species: Goat
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P
Related conjugates and formulations
概述
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产品名称
山羊抗大鼠IgG H&L (DyLight® 488)预吸附二抗
参阅全部 IgG 二抗 -
宿主
Goat -
靶标种属
Rat -
经测试应用
适用于: Flow Cyt, ICC/IF, IHC-Pmore details -
最小交叉反应
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep more details -
偶联物
DyLight® 488. Ex: 493nm, Em: 518nm
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488. -
克隆
多克隆 -
同种型
IgG -
研究领域
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab98420于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
1/50 - 1/200.
|
|
ICC/IF |
1/50 - 1/500.
|
|
IHC-P |
1/50 - 1/500.
|
说明 |
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Flow Cyt
1/50 - 1/200. |
ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. |
图片
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Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima. -
ICC/IF image of ab6161 stained Hela cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rat- H&L, pre-adsorbed (ab98420) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (5)
ab98420 被引用在 5 文献中.
- Zhang J et al. Giant proteins in a giant cell: Molecular basis of ultrafast Ca2+-dependent cell contraction. Sci Adv 9:eadd6550 (2023). PubMed: 36812318
- Yoo B et al. RNAi-Mediated PD-L1 Inhibition for Pancreatic Cancer Immunotherapy. Sci Rep 9:4712 (2019). PubMed: 30886310
- Yoo B et al. MicroRNA-710 regulates multiple pathways of carcinogenesis in murine metastatic breast cancer. PLoS One 14:e0226356 (2019). PubMed: 31834924
- Shrestha RL et al. TAO1 kinase maintains chromosomal stability by facilitating proper congression of chromosomes. Open Biol 4:130108 (2014). ICC/IF . PubMed: 24898139
- Liegel RP et al. Loss-of-function mutations in TBC1D20 cause cataracts and male infertility in blind sterile mice and Warburg micro syndrome in humans. Am J Hum Genet 93:1001-14 (2013). PubMed: 24239381