山羊多克隆抗体二抗to大鼠IgG - H&L (Cy3 ®),预吸附二抗
Chicken, Cow, Goat, Horse, Human, Mouse, Rabbit, Sheep
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.more details
Cy3 ®. Ex: 552nm, Em: 565nm
Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS
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Immunogen affinity purified
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3.
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/50 - 1/500.
||1/50 - 1/500.
||1/100 - 1/1000.
added following abreview 37065
Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3 ®), pre-adsorbed (ab98416)Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School
Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
- Fix in 3%PFA in PBS for 30 min at RT
- Incubate in 7.5% sucrose-PBS for 3h at RT
- Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
- Embed the EBs in tissue-Tek OCT compound
- Cut frozen sections to 4-20 µm thickness
Primary antibody: Rat anti-EndoA alpha 1, 1:100
Rabbit anti-laminin alpha 1, 1:400
Secondary antibody:Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3®) (ab98416), 1:100
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435
Nuclei were counterstained with SYTO 16
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"