山羊Anti-Rat IgG H&L (Cy3 ®) preadsorbed (ab6953)

概述

  • 产品名称山羊Anti-Rat IgG H&L (Cy3 ®) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to大鼠IgG - H&L (Cy3 ®), pre-adsorbed
  • 靶标种属Rat
  • 经测试应用适用于: ICC/IF, ICC, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details
  • 免疫原

    Full length native Rat IgG (purified).

  • 偶联物Cy3 ®. Ex: 552nm, Em: 565nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 8.5 moles Cy3 per mole of Goat IgG
  • 克隆多克隆
  • 同种型IgG
  • 常规说明This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6953 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

Goat Anti-Rat IgG H&L (Cy3 ®) preadsorbed (ab6953)参考文献

This product has been referenced in:
  • Stuckey DW  et al. Coordination of cell proliferation and anterior-posterior axis establishment in the mouse embryo. Development 138:1521-30 (2011). IHC (PFA fixed) . Read more (PubMed: 21427142) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"