山羊Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)

概述

  • 产品名称山羊Anti-Rat IgG Fc (DyLight® 550) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rat
  • 特异性By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to human and mouse IgG was detected.
  • 经测试应用适用于: IHC-P, ICC/IF, Flow Cytmore details
  • 最小交叉反应
    Human, Mouse more details
  • 偶联物DyLight® 550. Ex: 562nm, Em: 576nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • 克隆多克隆
  • 同种型IgG
  • 常规说明DyLight® 550 replaces DyLight® 549.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed 图像

  • Anti dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 550), pre-adsorbed (ab96972) was used as a secondary antibody at a concentration of 1/500.
  • Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells using Goat Anti-Rat IgG Fc (DyLight® 550) preabsorbed (ab96972) in yellow labelling a cell surface protein. Cells were incubated with the secondary antibody (1/500 in PBS + 2% BSA) for 1 hour at room temperature.

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)参考文献

ab96972 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Immunocytochemistry/ Immunofluorescence
yellow channel is anti-Rat Dy550, label a cell surface protein, no permeablization. work at dilution of 1:500, incubate in 2%BSA/PBS at RT for 1h
Username

Ms. YA ZHOU

Verified customer

提交于 Feb 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"