山羊Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)

概述

  • 产品名称山羊Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to兔IgG (PE), pre-adsorbed
  • 靶标种属Rabbit
  • 经测试应用适用于: Flow Cyt, ELISAmore details
  • 最小交叉反应
    Cow, Mouse more details
  • 免疫原

    Rabbit IgG

  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 1.64% Sodium phosphate, 1.71% Sucrose, 0.87% Sodium chloride, 0.1% BSA
  • Concentration information loading...
  • 纯度Size exclusion
  • 纯化说明Size exclusion chromatography as well as some proprietary methods for purifying the final materials were used.
  • 共轭说明Excitation max. l 565>498 nm. Emission max. l 578 nm. F/P 0.8:1.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab72465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt 1/500 - 1/1000.
ELISA Use at an assay dependent concentration.

Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed 图像

  • Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Phycoerythrin, pre-adsorbed) (ab72465) was used at 1/1000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 575/30 bandpass filter.

  • Sandwich ELISA to detect Rabbit IgG. Curve generated from a streptavidin-coated 96-well plate probed with dilutions of biotinylated Rabbit IgG and read after a 2 hour incubation at room temperature with ab72465

Goat Anti-Rabbit IgG H&L (Phycoerythrin) preadsorbed (ab72465)参考文献

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Thank you for contacting Abcam.

This product has not been tested in ICC and so while we cannot guarantee that it will work in that application, we see no reason to believe that it wouldn't. We would of course recommend thoroughly blocking yo...

Read More
Application Flow Cytometry
This secondary antibody was used to detect Slc4a1 on mouse erythrocytes as control as well as differentiated mouse embryonic stem cells. It can be seen in the picture attached that the labelling detected with this secondary antibody alone is practically identical to that obtained with un-labelled cells, meaning that the background is very low, which increases the confidence in the specificity of the primary antibody used.
Username

Alena Pance

Verified customer

提交于 Sep 08 2014

Thank you for contacting us today regarding secondary antibodies.

I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is ...

Read More

Thank you for your reply.
Below are the 2 products that I would recommend for you:
Isotype control - http://www.abcam.com/Rabbit-IgG-ChIP-Grade-ab37415.html
Secondary - http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H...

Read More

Thank you for contacting Abcam.
I would be happy to help you select the best products for your experiments. To be able to assist you further, would you please let me know which applications you wish to use these products in. That way I can recommen...

Read More

Thank you for your enquiry and interest in our products. In order to be able to search the compatible secondary antibodies, first  it is important to consider the isotypes of these two primary antibodies: ab9568 - mouse IgG1 kappa ab109022 - ra...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"