山羊抗兔IgG H&L (HRP)预吸附二抗(ab97080)

概述

  • 产品名称
    山羊抗兔IgG H&L (HRP)预吸附二抗
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to兔IgG - H&L (HRP),预吸附二抗
  • 宿主
    Goat
  • 靶标种属
    Rabbit
  • 特异性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
  • 经测试应用
    适用于: ICC, IHC-P, ELISA, WBmore details
  • 最小交叉反应

    Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details
  • 偶联物
    HRP

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. as primary.
WB 1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000.

图片

  • IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97080, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution developed using the ECL technique, with ab87342.

文献

This product has been referenced in:
  • Lu Y  et al. Cambogin suppresses dextran sulphate sodium-induced colitis by enhancing Treg cell stability and function. Br J Pharmacol 175:1085-1099 (2018). IHC-P ; Mouse . Read more (PubMed: 29352742) »
  • Cioni JM  et al. Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function. Neuron 97:1078-1093.e6 (2018). Read more (PubMed: 29518358) »

See all 20 Publications for this product

客户评价及客户问答

Application
Western blot
This item was applied as a secondary Ab for my WB. 1:10,000 dilution was applied for chemiluminescence reaction afterwards. I also conducted the procedure with secondary Ab probing only (right side of the blot) and the result showed no non-specific binding for this Ab. I am satisfied for this item in my WB assay.
Username

Abcam user community

Verified customer

提交于 Apr 11 2018

Application
Immunohistochemistry (Frozen sections)
Sections were thawed and fixed in cold acetone. Endogenous peroxidase and serum blocking (10% normal horse serum) were performed. Primary antibody was incubated with ab9722, at 5µg/ml overnight at 4°C. An HRP-conjugated secondary (ab97080 at 1/300 dilution) was used for 30min at room temperature. Sections were counterstained with haematoxylin and mounted with DPX.
A negative control was included using an isotype IgG at the same concentration as the primary antibody.
*Staining performed by Maria Wallert.
Username

Ana Maluenda

Verified customer

提交于 Jan 31 2018

Pré-adsorption est une étape supplémentaire pour augmenter la spécificité d'un anticorps. Je vous envoie un lien pour notre site internet qui explique ce terme en plus de détailes.

http://www.abcam.c...

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Thank you for contacting us.

Please have a look at the troubleshooting guide given on our website;

http://www.abcam.com/index.html?pageconfig=resource&rid=11384

I am sorry I am unable to get the specific protocol for chic...

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Thank you for your reply.

While I did not receive the image which you have referenced I would like to offer so help regarding your questions. Generally specific details regarding secondary antibody incubation and concentration as will need...

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Thank you for contacting us.

Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that sto...

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Thank you for your email.
I'm glad to see that the antibody worked.
Please do not hesitate to contact us if you need any more advice or information.
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Thank you for your email.
I'd suggest starting with the recommended dilutions for each antibody.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products...

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Thank you for your email.
The free of charge order was shipped today for delivery tomorrow.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Sub...

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You are very welcome. I keep my fingers crossed for you and wish you good luck.
Yes, definitely, please keep me informed. I look forward to hear back from you - hopefully with positive results.
Have a nice weekend.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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