山羊Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

概述

  • 产品名称山羊Anti-Rabbit IgG H&L (HRP) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属Rabbit
  • 经测试应用适用于: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Full length native Rabbit IgG (purified).

  • 偶联物HRP

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储溶液Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • 共轭说明Horseradish Peroxidase (HRP)
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA 1/20000 - 1/80000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed 图像

  • ab9986 staining osteoprotegerin in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA and 5% normal goat serum in PBS for 30 minutes at 22°C; Samples were incubated with primary antibody (1/20) for 9 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG H&L (ab7090) (1/300) was used as the secondary antibody.

    See Abreview

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)参考文献

This product has been referenced in:
  • Esbona K  et al. COX-2 modulates mammary tumor progression in response to collagen density. Breast Cancer Res 18:35 (2016). IHC-P . Read more (PubMed: 27000374) »
  • Gu L  et al. Rapamycin ameliorates CCl4-induced liver fibrosis in mice through reciprocal regulation of the Th17/Treg cell balance. Mol Med Rep 14:1153-61 (2016). IHC-P . Read more (PubMed: 27315465) »

See all 5 Publications for this product

Product Wall

Abreviews
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
This is an example of immunochemistry anti-Inos (ab15323) with Goat anti Rabbit HRP (on human artery)
-Deparaffinize
-heat in citrate buffer 20min at 96°C
-H2O2 for 10min at room temperature
-Block with PBS BSA 1% goat serum 5% at least 1h at room temperature
-incubate anti INOS at 1/100 in PBS BSA Overnight at 4°C
-incubate with goat anti rabbit HRP (1/300) for 45min at 37°C
-incubate slides in DAB or other peroxidase substrate
-counterstain with hematoxylin, dehydrate and mount
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Abcam user community

Verified customer

提交于 Jan 29 2014

Thank you for contacting us.

The Goat anti-rabbit HPR Conjugated used in AB80436 has been developed specifically for use in this kit and is currently not available for separate purchase. We do offer a number of Goat anti-rabbit secondaries...

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Thank you for your reply.

Reviewing the details, if you obtaining a band at 75 kDa in the no primary control with many secondary antibodies with the rat kidney lysate, but not the other samples, then I would recommend there is something in t...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these two secondaryantibody.

The details you have kindly provided will enable us to investigate this case for you and...

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This antibody has been pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This minimises the cross reactivity of this antibody with these proteins. However, minimal cross reactivity will still be ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"