山羊Anti-Rabbit IgG H&L (FITC) preadsorbed (ab7086)

概述

  • 产品名称
    山羊Anti-Rabbit IgG H&L (FITC) preadsorbed
    参阅全部 IgG 二抗
  • 靶标种属
    Rabbit
  • 经测试应用
    适用于: ICC/IF, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ELISAmore details
  • 最小交叉反应

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Full length native Rabbit IgG (purified).

  • 偶联物
    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 纯化说明
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • 共轭说明
    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab7086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF
Immunomicroscopy
Flow Cyt
IHC-P
IHC-Fr
ELISA
  • 应用说明
    Flow Cyt: Use at an assay dependant concentration.
    IHC-P: Use at an assay dependant concentration.
    IHC-Fr: Use at an assay dependant concentration.
    IM: Use at an assay dependant concentration.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat Anti-Rabbit IgG H&L (FITC) preadsorbed (ab7086)参考文献

    This product has been referenced in:
    • Wang H  et al. miR-33-5p, a novel mechano-sensitive microRNA promotes osteoblast differentiation by targeting Hmga2. Sci Rep 6:23170 (2016). Read more (PubMed: 26980276) »
    • Gasser A  et al. Activation of T cell calcium influx by the second messenger ADP-ribose. J Biol Chem 281:2489-96 (2006). ICC/IF . Read more (PubMed: 16316998) »

    See all 2 Publications for this product

    Product Wall

    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Used as part of an ISH/IHC double stain on fresh frozen human skeletal muscle. Used at a 1/200 dilution to bind to the Abcam Caspase-3 primary antibody (ab52293).

    Incubated at room temperature for 60 minutes in a dark staining chamber.
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    Abcam user community

    Verified customer

    提交于 Aug 01 2016

    Thank you for your inquiry.

    I can confirm that the ab47052 can be detected with this filter at 460 to 490nm. Indeed, the fluorochrome AMCA has a maximum emission at 445nm, however the fluorescent emission between 460 and 490nm should still be...

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    Thank you for your enquiry. First, when selecting a secondary antibody conjugated to a fluorochrome, you're limited by the available microscope filter sets that go with your microscope. Most filter sets are best matched with rhodamine or fluorescein. T...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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