山羊Anti-Rabbit IgG H&L (FITC) (ab97050)

概述

  • 产品名称
    山羊Anti-Rabbit IgG H&L (FITC)
    参阅全部 IgG 二抗
  • 靶标种属
    Rabbit
  • 特异性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • 经测试应用
    适用于: IHC-P, ICC/IF, Flow Cytmore details
  • 偶联物
    FITC. Ex: 493nm, Em: 528nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • 共轭说明
    F/P ratio is 4.85
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97050 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

图片

  • ab84235 staining melanoma inhibitory activity in a human melanoma cell line by Flow Cytometry. Cells were harvested with EDTA and washed in PBS. Cells were pemeabilized with saponine. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 20°C. A FITC-conjugated goat anti-rabbit IgG H&L (ab97050) (1/100) was used as the secondary antibody.

     

    See Abreview

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3% PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness

     



    Primary antibodies:
    Mouse anti-Ki67, 1:100
    Rabbit anti-laminin alpha 1 (basement marker)

    Secondary antibodies:
    Goat polyclonal Secondary Antibody to Mouse IgG - H&L (AMCA) (ab47052), 1:100
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050), 1:100

     

  • The FACS staining was perform on THP-1 cell lines with Rabbit polyclonal to CCR2 (ab21667) at a 1/100 dillution for 30 min at 4C. The secondary antibody was ab97050 used at 1/100 for 20min at 4C. The buffer used was PBS/BSA (0.5%)/Azide (0.05%). No fixation or permeabilization was performed and gating was done on alive cells.

    This image is courtesy of an anonymous Abreview

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3%PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab97050)
    F-actin was stained with CytoPainter F-actin staining kit (blue) (ab112124), 1:1000
    Nuclei were counterstained stained with DRAQ7TM (ab109202), 1:1000

  • The FACS staining was performed on HEK cells expressing human CCR5 with PBS/BSA (0.5%)/Azide (0.05%) as FACS buffer. We used a primary polyclonal rabbit antibody against CCR5 at a 1/100 dillution, incubated for 1 hour at 4C. The secondary antibody was ab97050 used at a concentration of 1/100 for 30min at 4C. No fixation or permeabilization was performed and gaiting was done on alive cells.

    This image is courtesy of an anonymous Abreview

文献

This product has been referenced in:
  • Yin SY  et al. Induction of IL-25 secretion from tumour-associated fibroblasts suppresses mammary tumour metastasis. Nat Commun 7:11311 (2016). Flow Cyt . Read more (PubMed: 27089063) »
  • More AS  et al. Prenatal Testosterone Exposure Leads to Gonadal Hormone-Dependent Hyperinsulinemia and Gonadal Hormone-Independent Glucose Intolerance in Adult Male Rat Offspring. Biol Reprod 94:5 (2016). IHC-P ; Rat . Read more (PubMed: 26586841) »

See all 10 Publications for this product

客户评价及客户问答

Thank you for contacting us.
According to the datasheet, the this protein is not expressed in most lymphoid cell lines: B-cells, T-cells and NK cells. Therefore, I would suggest to use a T-cell line like Jurkat lysate as a negative control.
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Thank you for your email and interest in Abcam products. We currently do not have AlexaFluor conjugated antibodies or proteins available in our catalog. Having said this though, we have a number of products available that are conjugated to DyLight® ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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