山羊Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96902)

Submit an Abreview Q&A (1)Specific References (2)
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概述

  • 产品名称
    山羊Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to兔IgG - H&L (DyLight® 650)， pre-adsorbed
  • 靶标种属
    Rabbit
  • 特异性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
  • 经测试应用
    适用于: IHC-P, ICC/IF, Flow Cytmore details
  • 最小交叉反应

    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • 偶联物
    DyLight® 650. Ex: 654nm, Em: 673nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明
    DyLight® 650 replaces DyLight® 649.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed 图像

  • DyLight®-Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 650), pre-adsorbed(ab96902)
    DyLight®-Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 650), pre-adsorbed(ab96902)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

Goat Anti-Rabbit IgG H&L (DyLight® 650) preadsorbed (ab96902)参考文献

This product has been referenced in:
  • Shenkman M  et al. A shared endoplasmic reticulum-associated degradation pathway involving the EDEM1 protein for glycosylated and nonglycosylated proteins. J Biol Chem 288:2167-78 (2013). ICC/IF . Read more (PubMed: 23233672) »
  • Mehmood R  et al. Cross-talk between distinct nuclear import pathways enables efficient nuclear import of E47 in conjunction with its partner transcription factors. Mol Biol Cell 22:3715-24 (2011). ICC/IF . Read more (PubMed: 21832153) »

See all 2 Publications for this product

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Votes: Highest First

Phone call regarding IHC-P staining of human samples with ab75850. Observing faint staining with background. Had been using 1/100 overnight at 4oC. Wants to optimise the protocol for double staining with ab377994.

As discussed over the phone I have looked at the protocols we have for double staining to see if I can suggest anything further to our conversation. As discussed the protocol that we have on our website is relatively simple, with sequential incubation ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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