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Our Abpromise guarantee covers the use of ab96886 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.|
|ICC/IF||1/50 - 1/500.|
|Flow Cyt||1/50 - 1/200.|
ab10559 staining CD45 in pig epithelial tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone. Samples were incubated with primary antibody (1/1000) for 12 hours at 4°C. A DyLight® 650-conjugated Goat anti-rabbit IgG H&L (ab96886) (1/500) was used as the secondary antibody.
ab96886 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"