山羊Anti-Rabbit IgG H&L (DyLight® 650) (ab96886)

概述

  • 产品名称
    山羊Anti-Rabbit IgG H&L (DyLight® 650)
    参阅全部 IgG 二抗
  • 靶标种属
    Rabbit
  • 特异性
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • 经测试应用
    适用于: IHC-P, ICC/IF, Flow Cytmore details
  • 偶联物
    DyLight® 650. Ex: 654nm, Em: 673nm

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 常规说明
    DyLight® 650 replaces DyLight® 649.
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab96886 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

图片

  • ab10559 staining CD45 in pig epithelial tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone. Samples were incubated with primary antibody (1/1000) for 12 hours at 4°C. A DyLight® 650-conjugated Goat anti-rabbit IgG H&L (ab96886) (1/500) was used as the secondary antibody.

    See Abreview

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

文献

ab96886 has not yet been referenced specifically in any publications.

客户评价及客户问答

excellent

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Good for internalization experiment, used at 1:500, red channel is surface GFP labelled in red, green channel is internalized GFP labelled with AB96886, it's invisible by eyes, but perfect for confocal imaging
Username

Ms. Ya Zhou

Verified customer

提交于 Oct 23 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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