山羊Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564)

概述

  • 产品名称山羊Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
    参阅全部 IgG 二抗
  • 描述
    山羊多克隆抗体二抗to兔IgG - H&L (Cy5 ®), pre-adsorbed
  • 靶标种属Rabbit
  • 经测试应用适用于: Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Frmore details
  • 最小交叉反应
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • 免疫原

    Rabbit IgG, whole molecule

  • 偶联物Cy5 ®. Ex: 650nm, Em: 667nm

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C.
  • 存储���液Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis.
  • 共轭说明Cy5.29 (Cyanine 5.29-OSu) (Molecular Weight 975 daltons) Absorption Wavelength: 650 nm Emission Wavelength: 667 nm Fluorochrome/Protein Ratio: 6.0 moles Cy5 per mole of Goat IgG
  • 克隆多克隆
  • 同种型IgG
  • 常规说明This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondaryantibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab6564 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
ELISA 1/10000 - 1/50000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed 图像

  • ab11175 staining Histone H2A.X in mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, and blocked with 4% BSA for 2 hours at 37°C. Antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/100 in PBS/4% BSA/0.05% Triton X-100) for 48 hours at 4°C. A Cy5®-conjugated goat anti-rabbit IgG H&L preadsorbed (ab6564) (1/300) was used as the secondary antibody.

    See Abreview

  • Ab6564 was used at dilution 1/300 with the primary antibody ab19898 in Flow Cyt. See the review on ab19898.
  • Ab6564 was used at dilution 1/500 with the primary antibody ab24586 in ICC/IF. See the review on ab24586.

Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab6564)参考文献

This product has been referenced in:
  • Liu H  et al. Curcumin suppresses transforming growth factor-ß1-induced cardiac fibroblast differentiation via inhibition of Smad-2 and p38 MAPK signaling pathways. Exp Ther Med 11:998-1004 (2016). ICC/IF . Read more (PubMed: 26998027) »
  • Azevedo AR  et al. Molecular Cytogenetics of Human Single Pronucleated Zygotes. Reprod Sci N/A:N/A (2014). Read more (PubMed: 24717739) »

See all 12 Publications for this product

Product Wall

Thank you for contacting us.

Both of these antibodies will be guaranteed for your purposes, and we would expect them to give equivalent signal and specificity. The main difference between the two is the recommended dilutions for ICC/IF. You s...

Read More

Thank you for your reply.

They could try the secondary ab6564 at 1/300 - 1/500.

http://www.abcam.com/Rabbit-IgG-secondary-antibody-H-L-ab6564.html#IgG-Secondary-antibodies-ab6564-1.jpg

I hope this information helps. Please...

Read More

Thank you for your inquiry.

We recommend using this antibody in FC 1"10 - 1:100 dilution. We tested permeabilized HeLa cells and the histograph can be found on the datasheet: http://www.abcam.com/Desmin-antibody-Y266-ab32362.html
Read More

Thank you for your email.

I can confirm that ab6564 is not yet tested experimentally inWB and therefore we cannot guarantee that ab6564 works in WB. But it is very likely to work in WB.

As my colleague who is not in the office today...

Read More

Thank you for your reply.

Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

As I pointed out before, the antibody is to be used in an untested application, you are eligible for...

Read More

Thank you very much for your interest in ab6564 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5 ®), pre-adsorbed.

To our knowledge, ab6564has not been tested in WB, and so has neither of our other secondary antibodies. Th...

Read More

Thank you very much for your interest in Abcam and our products..

To our knowledge,the following antibodies you listed have not been tested in IHC-P. Tested applications and species are listed on the individual datasheets. These are updated ...

Read More

Yes, the secondary antibody to Rabbit IgG - H&L (Cy5 ®) ab6564 is compatible with the anti-SAP102 antibody ab83980.

Thank you for your enquiry. Cy5 emits the color red (it is located at the Far-Red end of the spectrum). The following link on our website should be helpful for you (there is a Fluorochrome table on that particulat page): http://abcam2/index.html?pa...

Read More

Thank you for your email. Ab6075 has been tested for application in IHC-Frozen sections (IHC-Fr). It has not yet been tested for application in IHC-formalin fixed paraffin embedded tissue sections. For ab6564, Goat polyclonal to Rabbit IgG H&L (Cy5...

Read More

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"